In-situ growth of highly stable CsPbBr3@MSN@PbBr(OH) through water-assisted strategy for high sensitivity immunochromatographic assay

IF 13.3 1区 工程技术 Q1 ENGINEERING, CHEMICAL
Jie Liu, Tongxu Gu, Rui sun, Jiaojiao Sun, Jian Yin, Pengli Bai, Huancai Yin
{"title":"In-situ growth of highly stable CsPbBr3@MSN@PbBr(OH) through water-assisted strategy for high sensitivity immunochromatographic assay","authors":"Jie Liu, Tongxu Gu, Rui sun, Jiaojiao Sun, Jian Yin, Pengli Bai, Huancai Yin","doi":"10.1016/j.cej.2025.159861","DOIUrl":null,"url":null,"abstract":"Classical gold nanoparticle-based colorimetric immunochromatographic assay (ICA) exhibits moderate performance, limiting their application in trace detection. In this study, perovskite-MSN nanocomposites with high quantum yield (74.7 %), a narrow full width at half maximum (29.5 nm) and excellent water stability were synthesized using in situ crystallization growth and a water-assisted strategy. Carboxyl-modified perovskite-MSN nanocomposites were employed to label anti-gliadin monoclonal antibody (mAb) and construct dual-mode fluorescent ICA (FICA) for determination of gliadin in milk and beef samples. Compared to the traditional AuNP-based colorimetric ICA strips, the CsM-H-based FICA demonstrated improved sensitivity, with a limit of detection (LOD) of 3.62 ng/mL in quantitative analysis. The CsM-H-based FICA strips exhibited average recoveries ranging from 90.2 % to 110.0 %, with coefficients of variation (CVs) between 3.3 % and 7.4 % for milk and beef samples. The ultra-stable perovskite-MSN nanocomposites provided the FICA strip with excellent stability, enabling storage for up to six months at room temperature. This study highlights the advantages of CsM-H-based FICA, including its rapidity, simplicity, and cost-effectiveness, as a promising POCT biosensor.","PeriodicalId":270,"journal":{"name":"Chemical Engineering Journal","volume":"33 1","pages":""},"PeriodicalIF":13.3000,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical Engineering Journal","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1016/j.cej.2025.159861","RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, CHEMICAL","Score":null,"Total":0}
引用次数: 0

Abstract

Classical gold nanoparticle-based colorimetric immunochromatographic assay (ICA) exhibits moderate performance, limiting their application in trace detection. In this study, perovskite-MSN nanocomposites with high quantum yield (74.7 %), a narrow full width at half maximum (29.5 nm) and excellent water stability were synthesized using in situ crystallization growth and a water-assisted strategy. Carboxyl-modified perovskite-MSN nanocomposites were employed to label anti-gliadin monoclonal antibody (mAb) and construct dual-mode fluorescent ICA (FICA) for determination of gliadin in milk and beef samples. Compared to the traditional AuNP-based colorimetric ICA strips, the CsM-H-based FICA demonstrated improved sensitivity, with a limit of detection (LOD) of 3.62 ng/mL in quantitative analysis. The CsM-H-based FICA strips exhibited average recoveries ranging from 90.2 % to 110.0 %, with coefficients of variation (CVs) between 3.3 % and 7.4 % for milk and beef samples. The ultra-stable perovskite-MSN nanocomposites provided the FICA strip with excellent stability, enabling storage for up to six months at room temperature. This study highlights the advantages of CsM-H-based FICA, including its rapidity, simplicity, and cost-effectiveness, as a promising POCT biosensor.

Abstract Image

通过水辅助策略原位生长高度稳定的CsPbBr3@MSN@PbBr(OH),用于高灵敏度免疫层析分析
传统的基于金纳米粒子的比色免疫层析法(ICA)性能一般,限制了其在痕量检测中的应用。在本研究中,采用原位结晶生长和水辅助策略合成了高量子产率(74.7 %)、半宽窄(29.5 nm)和优异水稳定性的钙钛矿- msn纳米复合材料。采用羧基修饰的钙钛矿- msn纳米复合材料标记抗麦胶蛋白单克隆抗体(mAb),构建双模荧光ICA (FICA)检测牛奶和牛肉中的麦胶蛋白。与传统的基于aunp的比色ICA条相比,csm - h - FICA条的灵敏度更高,定量分析的检出限(LOD)为3.62 ng/mL。样品的平均回收率为90.2 % ~ 110.0 %,变异系数(cv)为3.3 % ~ 7.4 %。超稳定的钙钛矿- msn纳米复合材料为FICA带材提供了优异的稳定性,使其在室温下可储存长达6个月。本研究强调了基于csm - h的FICA的优势,包括其快速,简单和成本效益,作为一种有前途的POCT生物传感器。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Chemical Engineering Journal
Chemical Engineering Journal 工程技术-工程:化工
CiteScore
21.70
自引率
9.30%
发文量
6781
审稿时长
2.4 months
期刊介绍: The Chemical Engineering Journal is an international research journal that invites contributions of original and novel fundamental research. It aims to provide an international platform for presenting original fundamental research, interpretative reviews, and discussions on new developments in chemical engineering. The journal welcomes papers that describe novel theory and its practical application, as well as those that demonstrate the transfer of techniques from other disciplines. It also welcomes reports on carefully conducted experimental work that is soundly interpreted. The main focus of the journal is on original and rigorous research results that have broad significance. The Catalysis section within the Chemical Engineering Journal focuses specifically on Experimental and Theoretical studies in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. These studies have industrial impact on various sectors such as chemicals, energy, materials, foods, healthcare, and environmental protection.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信