Experimental Study on Myocardial Protective Effect of Xuebijing Injection on Extracorporeal Membrane Oxygenation Perfused Isolated Heart: Based on Autophagy and NLRP3 Inflammatory Pathway

IF 0.8 4区医学 Q4 IMMUNOLOGY

Transplantation proceedings Pub Date : 2025-01-21 DOI:10.1016/j.transproceed.2024.12.022

Shijie Yin , Chunhua Wang , Xiao Yue , Qiangxin Huang , Wei Wu , Guanbin Qin , Lan Luo , Huabei Wu

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引用次数: 0

Abstract

Background

The purpose of this study was to investigate the myocardial protective effect of Xuebijing (XBJ) injection in isolated donor heart preservation based on autophagy and NLRP3 inflammatory pathway, and to provide clues for improving the quality of donor heart preservation in the clinic.

Methods

Fourteen Guangxi Bama miniature pigs were randomly divided into two groups to establish the isolated heart perfusion model of extracorporeal membrane oxygenation (ECMO): (1) normal saline group (NS group): 50 mL normal saline was added to the perfusion solution; and (2) Xuebijing injection group (XBJ group): 10 mL of XBJ was added to the perfusate. Both groups were continuously pumped with 5 mL/h for 8 hours. Hemodynamic changes, inflammatory reaction, and myocardial enzyme levels were observed at five different time points. Western blot and real-time quantitative polymerase chain reaction (RT-qPCR) were used to detect the expression of autophagy markers and the NLRP3 signaling pathway related factors mRNA in myocardial tissue. Hematoxylin and eosin (H&E) staining and transmission electron microscopy were used to observe the pathomorphology and ultrastructure of the myocardium.

Results

There was no significant difference in perfusion pressure, heart rate, perfusion flow, and PH value between the two groups. The degree of myocardial tissue injury in the XBJ group was lighter, and the levels of myocardial enzymes, serum inflammatory factors were lower. The mRNA expression levels of beclin-1 and LC3 in the XBJ group were higher than those in the saline group, and the mRNA expression levels of NLRP3, Caspase-1, and ASC were lower.

Conclusions

Xuebijing injection can effectively improve the level of autophagy, reduce the activation and release of NLRP3 inflammasome, and slow down the inflammatory response, which has a certain myocardial protection effect.

查看原文本刊更多论文

血痹净注射液对离体心脏体外膜充氧灌注心肌保护作用的实验研究：基于自噬和NLRP3炎症通路。

背景：本研究旨在探讨血痹静注射液在基于自噬和NLRP3炎症通路的离体供心脏保存中的心肌保护作用，为临床提高供心脏保存质量提供线索。方法：将14头广西巴马小型猪随机分为2组，建立体外膜氧合（ECMO）离体心脏灌注模型：(1)生理盐水组（NS组）：灌注液中加入生理盐水50 mL；(2)血必经注射液组（XBJ组）：灌注液中加入XBJ 10 mL。两组均以5 mL/h连续泵送8 h。在5个不同时间点观察血流动力学变化、炎症反应和心肌酶水平。采用Western blot和实时定量聚合酶链反应（RT-qPCR）检测心肌组织中自噬标志物和NLRP3信号通路相关因子mRNA的表达。采用苏木精、伊红（H&E）染色和透射电镜观察心肌的病理形态学和超微结构。结果：两组大鼠灌注压、心率、灌注流量、PH值差异无统计学意义。XBJ组心肌组织损伤程度较轻，心肌酶、血清炎症因子水平较低。XBJ组beclin-1、LC3 mRNA表达水平高于生理盐水组，NLRP3、Caspase-1、ASC mRNA表达水平低于生理盐水组。结论：血必净注射液能有效提高大鼠自噬水平，降低NLRP3炎性体的激活和释放，减缓炎症反应，具有一定的心肌保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Transplantation proceedings 医学-免疫学

CiteScore

1.70

自引率

0.00%

发文量

502

审稿时长

60 days

期刊介绍： Transplantation Proceedings publishes several different categories of manuscripts, all of which undergo extensive peer review by recognized authorities in the field prior to their acceptance for publication. The first type of manuscripts consists of sets of papers providing an in-depth expression of the current state of the art in various rapidly developing components of world transplantation biology and medicine. These manuscripts emanate from congresses of the affiliated transplantation societies, from Symposia sponsored by the Societies, as well as special Conferences and Workshops covering related topics. Transplantation Proceedings also publishes several special sections including publication of Clinical Transplantation Proceedings, being rapid original contributions of preclinical and clinical experiences. These manuscripts undergo review by members of the Editorial Board. Original basic or clinical science articles, clinical trials and case studies can be submitted to the journal?s open access companion title Transplantation Reports.