A comparative study on in vitro models of necrotizing enterocolitis induced by single and combined stimulation

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology Pub Date : 2025-02-01 DOI:10.1016/j.ajg.2024.08.002

Chuchu Gao , Zongtai Feng , Lixia Wang , Kai Fu , Zuming Yang , Sannan Wang , Shenglin Yu

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引用次数: 0

Abstract

Background and study aims

Necrotizing enterocolitis (NEC) is a severe gastrointestinal disease in neonates. In vitro model is an indispensable tool to study the pathogenesis of NEC. This study explored the effects of different stress factors on intestinal injury in vitro.

Material and methods

Rat intestinal epithelial cell line-6 (IEC-6) cells were exposed to different stressors, including lipopolysaccharide (LPS), cobalt chloride (CoCl₂), and a combination of both. We estimated the cell viability, the expression of inflammatory cytokines and tight junction proteins.

Results

The decrease in IEC-6 cell viability was observed after stimulation by CoCl₂ alone or in combination with LPS, but not after stimulation with LPS alone. The expression of interleukin 6 (IL-6) and tumor necrosis factor alpha (TNFα) increased in each group. After stimulation with CoCl₂ alone or in combination with LPS, a decrease in Claudin-1 was observed, but an increase was detected after stimulation with LPS alone. Zona occludens 1 (ZO-1) decreased in both mRNA and protein levels after combined stimulation.

Conclusion

The combined stimulation of LPS and CoCl₂ on IEC-6 cells could simultaneously induce severe inflammation and barrier damage, which may better simulate the pathological process of NEC.

查看原文本刊更多论文

单一刺激与联合刺激诱导坏死性小肠结肠炎体外模型的比较研究。

背景与研究目的：坏死性小肠结肠炎（NEC）是新生儿中一种严重的胃肠疾病。体外模型是研究NEC发病机制不可缺少的工具。本研究探讨不同应激因素对体外肠道损伤的影响。材料和方法：将大鼠肠上皮细胞系6 （IEC-6）细胞暴露于不同的应激源，包括脂多糖（LPS）、氯化钴（CoCl2）以及两者的组合。我们检测了细胞活力、炎症因子和紧密连接蛋白的表达。结果：CoCl2单独刺激或LPS联合刺激后，IEC-6细胞活力下降，LPS单独刺激后无明显下降。各组白细胞介素6 （IL-6）、肿瘤坏死因子α (tnf - α)表达均升高。在CoCl2单独刺激或与LPS联合刺激后，观察到Claudin-1的下降，但在LPS单独刺激后检测到Claudin-1的增加。联合刺激后，闭塞带1 (ZO-1) mRNA和蛋白水平均降低。结论：LPS和CoCl2联合刺激IEC-6细胞可同时诱导严重炎症和屏障损伤，较好地模拟NEC的病理过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Arab Journal of Gastroenterology Medicine-Gastroenterology

CiteScore

2.70

自引率

0.00%

发文量

期刊介绍： Arab Journal of Gastroenterology (AJG) publishes different studies related to the digestive system. It aims to be the foremost scientific peer reviewed journal encompassing diverse studies related to the digestive system and its disorders, and serving the Pan-Arab and wider community working on gastrointestinal disorders.