[Genetic evolution analysis of enteric cytopathogenic human orphan virus type 30 in three cases of infants and young children in Hubei Province].

Q3 Medicine

中华预防医学杂志 Pub Date : 2025-01-06 DOI:10.3760/cma.j.cn112150-20240131-00103

K P Zhou, B Hu, K Pan, K Cai

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引用次数: 0

Abstract

Three throat swab samples from infants and young children in Hubei Province were identified as positive for enteroviruses. All subjects were one month old, and the onset of symptoms occurred in June; these cases were sporadic and exhibited no clear epidemiological associations. The results of VP1 gene amplification analysis indicated that all three infant cases were attributed to Echovirus 30 (ECHO30). The ECHO30 strain isolated from the three samples was classified as Cluster Ⅲ within the H gene subtype. Whole genome amplification analysis revealed that the ECHO30 strain from Hubei exhibited the highest homology with ECHO30/MW586892/Ashburton/17WQ2027G/USA/2007-08-20 from GenBank, demonstrating nucleotide and amino acid homologies of 97.6% and 99.3%, respectively. Recombination analysis indicated the presence of recombination events in the non-structural protein region of the ECHO30 Hubei strain, with recombinant fragments potentially originating from ECHO30/KY888274/14-397/Germany/2013 and ECHO18 strains Echo18/MN815811/BJ2018-S6363/Beijing/2018. This study provides a comprehensive analysis of the genetic evolution of the ECHO30 strain prevalent in Hubei Province, contributing essential data for the prevention of ECHO30 infections.

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[湖北省3例婴幼儿肠道细胞致病性人类孤儿病毒30型的遗传进化分析]。

湖北省3例婴幼儿咽拭子样本经鉴定为肠病毒阳性。所有受试者均为1月龄，症状出现于6月；这些病例是散发的，没有明显的流行病学关联。VP1基因扩增结果表明，3例患儿均为ECHO30病毒感染。从3份样本中分离到的ECHO30菌株属于H基因亚型簇Ⅲ。全基因组扩增结果显示，湖北ECHO30菌株与GenBank上的ECHO30/MW586892/Ashburton/17WQ2027G/USA/2007-08-20同源性最高，核苷酸和氨基酸同源性分别为97.6%和99.3%。重组分析表明，湖北株ECHO30的非结构蛋白区存在重组事件，重组片段可能来源于ECHO30/ ky888273 /14-397/德国/2013和ECHO18株ECHO18 /MN815811/BJ2018-S6363/北京/2018。本研究全面分析了湖北省流行的ECHO30菌株的遗传进化，为预防ECHO30感染提供了必要的数据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

中华预防医学杂志 Medicine-Medicine (all)

CiteScore

1.20

自引率

0.00%

发文量

12678

期刊介绍： Chinese Journal of Preventive Medicine (CJPM), the successor to Chinese Health Journal , was initiated on October 1, 1953. In 1960, it was amalgamated with the Chinese Medical Journal and the Journal of Medical History and Health Care , and thereafter, was renamed as People’s Care . On November 25, 1978, the publication was denominated as Chinese Journal of Preventive Medicine . The contents of CJPM deal with a wide range of disciplines and technologies including epidemiology, environmental health, nutrition and food hygiene, occupational health, hygiene for children and adolescents, radiological health, toxicology, biostatistics, social medicine, pathogenic and epidemiological research in malignant tumor, surveillance and immunization.