Exosomal miR-27a-5p attenuates inflammation through Toll-like receptor 7 in foodborne Salmonella infections

IF 2.4 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology Pub Date : 2025-01-14 DOI:10.1016/j.vetmic.2025.110394

Mingjuan Qu , Shengfa Su , Linlin Jiang , Xin Yu , Jianlong Zhang , Hongwei Zhu , Kexue Han , Xingxiao Zhang

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引用次数: 0

Abstract

Salmonella is a common food-borne pathogen that is highly pathogenic and infectious, causing serious harm to livestock breeding and food safety. Uncovering the mechanisms of Salmonella infection and immune evasion can effectively prevent Salmonella contamination of livestock and poultry food. Here, small RNA sequencing results showed that exosomes produced by naïve murine macrophages RAW 264.7 cells contained a unique enrichment of a set of microRNAs (miRNAs) after Salmonella infection. Quantitative real-time polymerase chain reaction (qPCR) analysis verified that the tested miRNA (i.e. miR-27a-5p, miR-92a-1-5p and miR-1249-5p) showed similar expression patterns, consistent with small RNA sequencing data. TargetScan database predicted that the most promising targets for the differentially expressed miRNAs were abundant in the immune system, infectious diseases, and signal transduction pathways. Dual-luciferase reporter assays confirmed that Toll-like receptor 7 (TLR7) was the target of miR-27a-5p. Western blotting and enzyme-linked immunosorbent assay (ELISA) results revealed that overexpression of miR-27a-5p suppressed inflammation by targeting TLR7/nuclear factor kappa-B (NF-κB) signaling pathway and leading interleukin-6 (IL-6) and IL-1β cytokines slightly reduction in recipient macrophages, suggesting that exosomal miR-27a-5p uptake by naïve macrophages may inhibit pro-inflammatory macrophage differentiation. Therefore, these results contribute to our systematic understanding of the mechanism of exosomal miRNA in Salmonella infection, providing a potential target for preventing immune escape from Salmonella.

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在食源性沙门氏菌感染中，外泌体miR-27a-5p通过toll样受体7减轻炎症。

沙门氏菌是一种常见的食源性致病菌，具有高致病性和传染性，对家畜养殖和食品安全造成严重危害。揭示沙门氏菌感染和免疫逃逸的机制，可以有效预防沙门氏菌污染畜禽食品。这里，小RNA测序结果显示naïve小鼠巨噬细胞RAW 264.7细胞产生的外泌体在沙门氏菌感染后含有一组独特的microRNAs （miRNAs）富集。定量实时聚合酶链反应（qPCR）分析证实，检测的miRNA（即miR-27a-5p， miR-92a-1-5p和miR-1249-5p）表现出相似的表达模式，与小RNA测序数据一致。TargetScan数据库预测，这些差异表达的mirna最有希望的靶标在免疫系统、传染病和信号转导途径中丰富。双荧光素酶报告基因检测证实toll样受体7 （TLR7）是miR-27a-5p的靶标。Western blotting和酶联免疫吸附试验（ELISA）结果显示，过表达miR-27a-5p通过靶向TLR7/核因子κ b （NF-κB）信号通路和导致受体巨噬细胞中白细胞介素-6 （IL-6）和IL-1β细胞因子的轻微降低来抑制炎症，提示naïve巨噬细胞摄取外泌体miR-27a-5p可能抑制促炎巨噬细胞的分化。因此，这些结果有助于我们系统地了解外泌体miRNA在沙门氏菌感染中的作用机制，为防止沙门氏菌的免疫逃逸提供潜在的靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary microbiology 农林科学-兽医学

CiteScore

5.90

自引率

6.10%

发文量

221

审稿时长

52 days

期刊介绍： Veterinary Microbiology is concerned with microbial (bacterial, fungal, viral) diseases of domesticated vertebrate animals (livestock, companion animals, fur-bearing animals, game, poultry, fish) that supply food, other useful products or companionship. In addition, Microbial diseases of wild animals living in captivity, or as members of the feral fauna will also be considered if the infections are of interest because of their interrelation with humans (zoonoses) and/or domestic animals. Studies of antimicrobial resistance are also included, provided that the results represent a substantial advance in knowledge. Authors are strongly encouraged to read - prior to submission - the Editorials (''Scope or cope'' and ''Scope or cope II'') published previously in the journal. The Editors reserve the right to suggest submission to another journal for those papers which they feel would be more appropriate for consideration by that journal. Original research papers of high quality and novelty on aspects of control, host response, molecular biology, pathogenesis, prevention, and treatment of microbial diseases of animals are published. Papers dealing primarily with immunology, epidemiology, molecular biology and antiviral or microbial agents will only be considered if they demonstrate a clear impact on a disease. Papers focusing solely on diagnostic techniques (such as another PCR protocol or ELISA) will not be published - focus should be on a microorganism and not on a particular technique. Papers only reporting microbial sequences, transcriptomics data, or proteomics data will not be considered unless the results represent a substantial advance in knowledge. Drug trial papers will be considered if they have general application or significance. Papers on the identification of microorganisms will also be considered, but detailed taxonomic studies do not fall within the scope of the journal. Case reports will not be published, unless they have general application or contain novel aspects. Papers of geographically limited interest, which repeat what had been established elsewhere will not be considered. The readership of the journal is global.