Effectiveness of a single-dose phage cocktail on the reduction of multidrug-resistant Escherichia coli in suckling piglets

IF 2.4 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology Pub Date : 2025-01-13 DOI:10.1016/j.vetmic.2025.110395

Viphavanh Chanthavong , Nattha Vigad , Wattana Pelyuntha , David Yembilla Yamik , Kitiya Vongkamjan , Mingkwan Yingkajorn , Warangkhana Chaisowwong , Kittiphong Tippaya , Phacharaporn Tadee , Kridda Chukiatsiri

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Abstract

Antibiotics are commonly used in pig farming to control infections caused by diarrhea-causing Escherichia coli (E. coli). However, improper or excessive use of antibiotics in pigs can enhance antibiotic resistance (ABR). This study used bacteriophage (phage) treatment to control ABR E. coli in diarrheal suckling piglets. Fifty E. coli isolates were previously isolated from suckling pigs, which showed resistance to amoxicillin (100 %), oxytetracycline and neomycin (94 %), sulfamethoxazole-trimethoprim (70 %), gentamicin (56 %), cephalexin (54 %), enrofloxacin (42 %), and colistin (28 %). Five phages (WPEC1, WPEC2, WPEC3, WPEC4, and WPEC5) were included in this study. These phages showed a diverse lytic profile ranging from 46.0 % to 64.0 % on the tested ABR E. coli isolates. The phage cocktail reduced the count of five representative E. coli by showing up to 8 log-units reduction (p < 0.05) after phage treatment for 6–24 h. From the in vivo study, a single dose of the phage cocktail (9 log PFU/mL) reduced the number of E. coli present in the gastrointestinal tract of suckling piglets by showing a 1.33 log-units reduction on day 7 (p < 0.05). In addition, the fecal score of the phage treatment group was lower than that of the control group (p < 0.05). However, body weight gain (BWG) and average daily gain (ADG) were not significantly different in both groups (p > 0.05). These findings suggest that a developed phage cocktail could be used as a potential biocontrol to fight ABR E. coli, reduce the chance of piglet mortality, and increase safety during pig production.

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单剂量噬菌体鸡尾酒对减少哺乳仔猪多重耐药大肠杆菌的效果。

抗生素通常用于养猪业，以控制由引起腹泻的大肠杆菌引起的感染。然而，不适当或过量使用抗生素会增强猪的抗生素耐药性。本研究采用噬菌体（phage）处理控制腹泻哺乳仔猪ABR大肠杆菌。先前从乳猪中分离出50株大肠杆菌，对阿莫西林（100 %）、土霉素和新霉素（94 %）、磺胺甲氧唑-甲氧苄啶（70 %）、庆大霉素（56 %）、头孢氨苄（54 %）、恩诺沙星（42 %）和粘菌素（28 %）耐药。本研究共纳入5个噬菌体（WPEC1、WPEC2、WPEC3、WPEC4、WPEC5）。这些噬菌体在ABR大肠杆菌分离株上显示出不同的裂解谱，范围从46.0 %到64.0 %。噬菌体鸡尾酒减少了5个代表性大肠杆菌的计数，减少了8个对数单位（p  0.05）。这些发现表明，开发出的噬菌体鸡尾酒可作为一种潜在的生物防治手段来对抗ABR大肠杆菌，降低仔猪死亡率，提高生猪生产过程中的安全性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary microbiology 农林科学-兽医学

CiteScore

5.90

自引率

6.10%

发文量

221

审稿时长

52 days

期刊介绍： Veterinary Microbiology is concerned with microbial (bacterial, fungal, viral) diseases of domesticated vertebrate animals (livestock, companion animals, fur-bearing animals, game, poultry, fish) that supply food, other useful products or companionship. In addition, Microbial diseases of wild animals living in captivity, or as members of the feral fauna will also be considered if the infections are of interest because of their interrelation with humans (zoonoses) and/or domestic animals. Studies of antimicrobial resistance are also included, provided that the results represent a substantial advance in knowledge. Authors are strongly encouraged to read - prior to submission - the Editorials (''Scope or cope'' and ''Scope or cope II'') published previously in the journal. The Editors reserve the right to suggest submission to another journal for those papers which they feel would be more appropriate for consideration by that journal. Original research papers of high quality and novelty on aspects of control, host response, molecular biology, pathogenesis, prevention, and treatment of microbial diseases of animals are published. Papers dealing primarily with immunology, epidemiology, molecular biology and antiviral or microbial agents will only be considered if they demonstrate a clear impact on a disease. Papers focusing solely on diagnostic techniques (such as another PCR protocol or ELISA) will not be published - focus should be on a microorganism and not on a particular technique. Papers only reporting microbial sequences, transcriptomics data, or proteomics data will not be considered unless the results represent a substantial advance in knowledge. Drug trial papers will be considered if they have general application or significance. Papers on the identification of microorganisms will also be considered, but detailed taxonomic studies do not fall within the scope of the journal. Case reports will not be published, unless they have general application or contain novel aspects. Papers of geographically limited interest, which repeat what had been established elsewhere will not be considered. The readership of the journal is global.