Evaluating the In Vitro Anthelmintic Activity of Zinc Oxide Nanoparticles (ZnO-NPs) Against the Adult Stages of Fasciola hepatica.

IF 1.8 3区农林科学 Q2 VETERINARY SCIENCES

Veterinary Medicine and Science Pub Date : 2025-01-01 DOI:10.1002/vms3.70170

Mahmoud Abdelhamid, Dalia Fouad, Abdallah Alian, Asmaa A Nasr, H Abd-Allah, Mohamed Farouk, Barakat Shehata Abd Elmaleck, Athanasios Alexiou, Marios Papadakis, Gaber El-Saber Batiha, Fatma A S Anwar

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引用次数: 0

Abstract

Background: Fascioliasis represents one of the most significant parasitic and foodborne zoonotic diseases in the world. Resistance to currently deployed human and veterinary flukicides is a growing health problem. Zinc oxide nanoparticles (ZnO-NPs) have developed enormous importance in nanomedicine. The current study aims to evaluate the flukicide activity of ZnO-NPs on the adult stages of Fasciola hepatica, in vitro assay.

Methods: Mature flukes of F. hepatica were collected from the biliary tracts of cattle liver from the main slaughterhouses in Aswan Governorate, Egypt. The co-precipitation process was utilized to create ZnO-NPs. The adult active flukes of F. hepatica (n = 6) were transferred in Petri dishes (triplicates) containing different concentrations of ZnO-NPs (0.5, 0.10 and 0.12 mg/mL), with simultaneous maintenance of control in only PBS. The treated and control groups of flukes were maintained inside an incubator at 37°C ± 1°C. Mortality and paralysis of the flukes were observed. The adult flukes were picked up for scanning electron microscopy (SEM) examination from the control medium, and those exposed to the highest effective concentration of ZnO-NPs. ZnO-NPs showed dose-dependent flukicide efficacy against the adult flukes.

Results: At 0.12 mg/mL concentration of ZnO-NPs, the mortality of flukes was attained early in 4.05 ± 0.033 h. The photomicrographs obtained by SEM of the flukes revealed that ZnO-NPs (0.12 mg/mL) had a potent effect on the integument surface and genital system of F. hepatica. Most of the tegument was damaged with complete erosion and loss of the spines and papillae. Furthermore, a severe rupture in the genital system causes the uterus to burst and releases eggs through the genital pore.

Conclusions: Hence, it could be concluded that ZnO-NPs performed effective anthelmintic activity against the adult stages of F. hepatica, in vitro. The results of the present study recommend the use of ZnO-NPs as new preparations with anthelmintic activity.

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氧化锌纳米颗粒（ZnO-NPs）对肝片形吸虫成虫的体外驱虫活性评价。

背景：片形吸虫病是世界上最重要的寄生虫和食源性人畜共患疾病之一。对目前使用的人类和兽医杀菌剂的耐药性是一个日益严重的健康问题。氧化锌纳米颗粒（ZnO-NPs）在纳米医学中具有重要的应用价值。本研究旨在通过体外实验评估ZnO-NPs对肝片形吸虫成虫期的杀流活性。方法：从埃及阿斯旺省主要屠宰场的牛肝脏胆道中采集成熟的肝吸虫。采用共沉淀法制备ZnO-NPs。将6只肝炎F.活动性成年吸虫（n = 6）转移到含有不同浓度ZnO-NPs（0.5、0.10和0.12 mg/mL）的培养皿（3个重复）中，同时只在PBS中维持对照。处理组和对照组吸虫置于37℃±1℃培养箱内。观察了吸虫的死亡和麻痹。从对照培养基和暴露于ZnO-NPs有效浓度最高的培养基中，采集成年吸虫进行扫描电镜（SEM）观察。ZnO-NPs对成年吸虫的杀虫效果呈剂量依赖性。结果：在0.12 mg/mL的ZnO-NPs浓度下，吸虫早于4.05±0.033 h死亡，扫描电镜观察发现，0.12 mg/mL的ZnO-NPs浓度对肝f.s的被膜表面和生殖系统有明显的影响。大部分被皮受损，完全糜烂，失去了棘和乳头。此外，严重的生殖系统破裂会导致子宫破裂，并通过生殖孔释放卵子。结论：ZnO-NPs在体外对肝单胞菌成虫具有较强的驱虫活性。本研究结果推荐ZnO-NPs作为具有驱虫活性的新制剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary Medicine and Science Veterinary-General Veterinary

CiteScore

3.00

自引率

0.00%

发文量

296

期刊介绍： Veterinary Medicine and Science is the peer-reviewed journal for rapid dissemination of research in all areas of veterinary medicine and science. The journal aims to serve the research community by providing a vehicle for authors wishing to publish interesting and high quality work in both fundamental and clinical veterinary medicine and science. Veterinary Medicine and Science publishes original research articles, systematic reviews, meta-analyses, and research methods papers, along with invited editorials and commentaries. Original research papers must report well-conducted research with conclusions supported by the data presented in the paper. We aim to be a truly global forum for high-quality research in veterinary medicine and science, and believe that the best research should be published and made widely accessible as quickly as possible. Veterinary Medicine and Science publishes papers submitted directly to the journal and those referred from a select group of prestigious journals published by Wiley-Blackwell. Veterinary Medicine and Science is a Wiley Open Access journal, one of a new series of peer-reviewed titles publishing quality research with speed and efficiency. For further information visit the Wiley Open Access website.