Genetic diversity and antigenic variability of foot-and-mouth disease virus strains in Egypt.

IF 1.7 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Hosein I Hosein, Khaled Abosria, Sherin R Rouby, El-Shaymaa El-Nahass, Asmaa L A Zin Eldin, Samar S Ewies, Ahmed S Abdel-Moneim
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Abstract

Foot-and-mouth disease (FMD) continues to pose a significant threat in Egypt, necessitating thorough analyses of FMD virus (FMDV) outbreaks. This study analyzed 144 suspected FMD cases across 52 animal collections during the years 2017-2018 and 2022. Recurrent FMD outbreaks in vaccinated dairy cattle were investigated. Clinical observation, postmortem examination, and histopathological analysis were conducted. Samples were subjected to reverse transcription polymerase chain reaction (RT-PCR) using group-specific in addition to serotype-specific primers, along with direct gene sequencing of the VP1 gene from positive samples, followed by phylogenetic analysis. Signs, postmortem and histopathological lesions consistent with FMD were found. Molecular identification confirmed the presence of serotype A (G-IV topotype), O (O/EA3 topotype), and serotype SAT2 (SAT2/VII/Lib-03 topotype). All detected topotypes were distinct from currently available vaccine strains. Variations in amino acid in the epitopes of the structural protein VP1 were detected. The study highlights the genetic and antigenic variability of FMDV strains circulating in Egypt, indicating potential challenges for vaccine effectiveness.

埃及口蹄疫病毒株的遗传多样性和抗原性变异
口蹄疫继续对埃及构成重大威胁,有必要对口蹄疫病毒暴发进行彻底分析。本研究分析了2017-2018年和2022年期间采集的52只动物中的144例疑似口蹄疫病例。对接种过疫苗的奶牛中口蹄疫的反复暴发进行了调查。进行临床观察、尸检和组织病理学分析。使用群体特异性和血清型特异性引物对样本进行逆转录聚合酶链反应(RT-PCR),同时对阳性样本的VP1基因进行直接基因测序,然后进行系统发育分析。发现了与口蹄疫相符的体征、死后和组织病理学病变。分子鉴定证实存在血清型A (G-IV型)、O (O/EA3型)和血清型SAT2 (SAT2/VII/Lib-03型)。所有检测到的拓扑型都不同于目前可用的疫苗株。在结构蛋白VP1的表位上检测到氨基酸的变异。该研究强调了在埃及流行的口蹄疫病毒毒株的遗传和抗原变异,表明疫苗有效性面临潜在挑战。
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来源期刊
Tropical animal health and production
Tropical animal health and production 农林科学-兽医学
CiteScore
3.40
自引率
11.80%
发文量
361
审稿时长
6-12 weeks
期刊介绍: Tropical Animal Health and Production is an international journal publishing the results of original research in any field of animal health, welfare, and production with the aim of improving health and productivity of livestock, and better utilisation of animal resources, including wildlife in tropical, subtropical and similar agro-ecological environments.
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