Transcription factors PHR1 and PHR1-like 1 regulate ABA-mediated inhibition of seed germination and stomatal opening in Arabidopsis

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-01-16 DOI:10.1016/j.plantsci.2025.112389

Huiying Chen , Jia Du , Yifan Wang , Kexin Chao , Zitong Wang , Shahid Ali , Houqing Zeng

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引用次数: 0

Abstract

Low phosphate (LP) availability significantly impacts crop yield and quality. PHOSPHATE STARVATION RESPONSE1 (PHR1) along with PHR1-like 1 (PHL1) act as a key transcriptional regulator in a plant's adaptive response to LP conditions. Abscisic acid (ABA) plays an important role in how plants respond to environmental stresses like salinity and drought. However, the involvement of PHR1 and PHL1 in ABA response and signalling mechanisms remains to be fully understood. Our findings reveal that PHR1 and PHR1/PHL1 knockout mutations enhance the responsiveness of seed germination, early seedling growth, and stomatal opening to ABA in Arabidopsis. Furthermore, these mutations increase sensitivity to combined LP and ABA stress. In contrast, overexpression of PHR1 or PHL1 reduces this sensitivity in Arabidopsis. Knockout mutations of PHR1 and PHR1/PHL1 also increase sensitivity to salt and osmotic stresses, as well as to combined LP and salinity/osmotic stress, while overexpression of PHR1 or PHL1 reduces their sensitivity in seed germination and early seedling development. Knockout mutations of SPX1 and SPX2, negative regulators of PHR1 and PHL1, decrease sensitivity to ABA and salt/osmotic stresses in Arabidopsis. A group of genes related to ABA metabolism and signalling is significantly affected by the knockout or overexpression of PHR1 and PHL1, with a large proportion of these genes containing PHR1 binding site (P1BS) in their promoters. Moreover, the ABA-sensitive phenotype of phr1 or phr1 phl1 mutants can be rescued by PHR1 homologs from chlorophyte algae, liverwort and rice, suggesting their conserved roles in ABA signalling. These results indicate that PHR1 and its homologs negatively regulate plant responses to ABA in seed germination and stomatal aperture. This study provides new insights into the interplay between Pi homeostasis, abiotic stress and ABA signaling. Moderately increasing the expression of PHR1 or its homologs in crops could be a potential strategy to enhance plant resistance to combined LP and osmotic stress.

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转录因子PHR1和PHR1样1调节aba介导的拟南芥种子萌发和气孔打开的抑制作用。

磷素效价低严重影响作物产量和品质。磷酸盐饥饿应答1 （PHR1）和PHR1样1 （PHL1）在植物对LP条件的适应性反应中起关键的转录调节作用。脱落酸（ABA）在植物对盐度和干旱等环境胁迫的反应中起着重要作用。然而，PHR1和PHL1参与ABA反应和信号传导机制仍有待充分了解。研究结果表明，PHR1和PHR1/PHL1敲除突变增强了拟南芥种子萌发、幼苗早期生长和气孔开放对ABA的响应性。此外，这些突变增加了对LP和ABA联合胁迫的敏感性。相反，在拟南芥中，PHR1或PHL1的过表达降低了这种敏感性。PHR1和PHR1/PHL1的敲除突变也增加了对盐和渗透胁迫的敏感性，以及对LP和盐/渗透胁迫的敏感性，而PHR1或PHL1的过表达降低了它们在种子萌发和幼苗早期发育中的敏感性。SPX1和SPX2是PHR1和PHL1的负调节因子，敲除突变会降低拟南芥对ABA和盐/渗透胁迫的敏感性。PHR1和PHL1的敲除或过表达会显著影响ABA代谢和信号传导相关的一组基因，其中很大一部分基因的启动子中含有PHR1结合位点（P1BS）。此外，phr1或phr1 phl1突变体的ABA敏感表型可以被来自绿藻、苔类和水稻的phr1同源物拯救，这表明它们在ABA信号传导中具有保守作用。上述结果表明，PHR1及其同源基因负调控植物种子萌发和气孔孔径对ABA的响应。该研究为Pi稳态、非生物胁迫和ABA信号传导之间的相互作用提供了新的见解。适度增加PHR1或其同源物在作物中的表达可能是提高植物对LP和渗透胁迫联合抗性的潜在策略。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.