Effectiveness of salt priming and plant growth-promoting bacteria in mitigating salt-induced photosynthetic damage in melon.

Abstract

Seed priming and plant growth-promoting bacteria (PGPB) may alleviate salt stress effects. We exposed a salt-sensitive variety of melon to salinity following seed priming with NaCl and inoculation with Bacillus. Given the sensitivity of photosystem II (PSII) to salt stress, we utilized dark- and light-adapted chlorophyll fluorescence alongside analysis of leaf stomatal conductance of water vapour (G_sw). Priming increased total seed germination by 15.5% under salt-stress. NaCl priming with Bacillus inoculation (PB) increased total leaf area (LA) by 45% under control and 15% under stress. Under the control condition, priming (P) reduced membrane permeability (RMP) by 36% and PB by 55%, while under stress Bacillus (BS) reduced RMP by 10%. Although Bacillus inoculation (B) and priming (P) treatments did not show significant effects on some PSII efficiency parameters (F_V/F_M, ABS/RC, PI_ABS, F_M), the BS treatment induced a significantly higher quantum efficiency of PSII (ΦPSII) and increased G_sw by 159% in the final week of the experiment. The BS treatment reduced electron transport rate per reaction center (ET_O/RC) by 10% in comparison to the salt treatment, which showed less reaction centre damage. Bacillus inoculation and seed priming treatment under the stressed condition (PBS) induced an increase in electron transport rate of 40%. Salt stress started to show significant effects on PSII after 12 days, and adversely impacted all morphological and photosynthetic parameters after 22 days. Salt priming and PGPB mitigated the negative impacts of salt stress and may serve as effective tools in future-proofing saline agriculture.