Epidemiology and Genotype Dynamics of Dengue in Hospitalized Patients in Northern Vietnam Between 2020 and 2022.

IF 3.8 4区 医学 Q2 IMMUNOLOGY
Open Forum Infectious Diseases Pub Date : 2024-12-24 eCollection Date: 2025-01-01 DOI:10.1093/ofid/ofae753
Do Duc Anh, Nguyen Trong The, Truong Nhat My, Le Thi Kieu Linh, Nghiem Xuan Hoan, Peter G Kremsner, Nguyen Linh Toan, Le Huu Song, Thirumalaisamy P Velavan
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引用次数: 0

Abstract

Background: Arboviruses, including Dengue (DENV), Zika, and chikungunya, cause recurrent outbreaks of varying intensity in tropical countries. This study aimed to investigate other arboviruses, including Zika and chikungunya, in patients clinically suspected of Dengue and to characterize the circulating Dengue serotypes and genotypes in Northern Vietnam from 2020 to 2022. To date, information on this topic in the region has been limited.

Methods: Multiplex real-time polymerase chain reaction (PCR) was used to detect Dengue, Zika, and chikungunya RNA, and DENV serotypes were identified via real-time reverse transcriptase PCR from 426 clinically Dengue suspected patients. Patients were screened for NS1 antigen and anti-DENV immunoglobulin (Ig) G/IgM antibodies. Phylogenetic analysis of DENV Capsid-premembrane gene sequences was performed to investigate genotype distribution.

Results: Dengue was confirmed in 95% of cases, with no Zika or chikungunya RNA detected. DENV-2 was the predominant serotype (61%), followed by DENV-1 (31%) and DENV-4 (7%). Coinfections were observed, with DENV-1 and DENV-2 being the most common. In 2022, a high incidence of Dengue cases with warning signs and severe Dengue was observed, accompanied by elevated liver enzyme levels and reduced platelet counts. Phylogenetic analysis revealed that the DENV-1 and DENV-4 serotypes clustered with previously reported regional virus, while DENV-2 showed a shift from genotype Asian I to Cosmopolitan over the study period.

Conclusions: This study underscores a significant rise in Dengue severity and shifts in DENV genotypes in recent years in Northern Vietnam, emphasizing the importance of understanding genotype dynamics and clinical dynamics for improving outbreak preparedness and response strategies.

2020 - 2022年越南北部住院患者登革热流行病学和基因型动态
背景:虫媒病毒,包括登革热(DENV)、寨卡病毒和基孔肯雅热,在热带国家引起不同强度的反复暴发。本研究旨在调查临床疑似登革热患者感染的其他虫媒病毒,包括寨卡病毒和基孔肯雅病毒,并分析2020 - 2022年越南北部登革热流行血清型和基因型特征。迄今为止,该区域关于这一专题的资料有限。方法:采用多重实时聚合酶链反应(PCR)检测登革热、寨卡病毒和基孔肯雅病毒RNA,并通过实时逆转录酶PCR对426例临床登革热疑似患者进行DENV血清型鉴定。筛查患者NS1抗原和抗denv免疫球蛋白(Ig) G/IgM抗体。对DENV衣壳膜前基因序列进行系统发育分析,探讨DENV的基因型分布。结果:95%的病例确诊为登革热,未检出寨卡病毒或基孔肯雅病毒RNA。DENV-2是主要血清型(61%),其次是DENV-1(31%)和DENV-4(7%)。观察到共感染,以DENV-1和DENV-2最常见。2022年,观察到具有警告信号和严重登革热的登革热病例高发,并伴有肝酶水平升高和血小板计数减少。系统发育分析显示,DENV-1和DENV-4血清型与先前报道的区域性病毒聚集在一起,而DENV-2在研究期间从亚洲I型基因型向世界型基因型转变。结论:本研究强调了近年来越南北部登革热严重程度的显著上升和DENV基因型的变化,强调了了解基因型动力学和临床动力学对于改进疫情准备和应对策略的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Open Forum Infectious Diseases
Open Forum Infectious Diseases Medicine-Neurology (clinical)
CiteScore
6.70
自引率
4.80%
发文量
630
审稿时长
9 weeks
期刊介绍: Open Forum Infectious Diseases provides a global forum for the publication of clinical, translational, and basic research findings in a fully open access, online journal environment. The journal reflects the broad diversity of the field of infectious diseases, and focuses on the intersection of biomedical science and clinical practice, with a particular emphasis on knowledge that holds the potential to improve patient care in populations around the world. Fully peer-reviewed, OFID supports the international community of infectious diseases experts by providing a venue for articles that further the understanding of all aspects of infectious diseases.
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