{"title":"B-doped nano-hydroxyapatite facilitates proliferation and differentiation of osteoblasts.","authors":"Qiao Kang, Wenhao Wang, Shengtao Wu, Ganggang Hu","doi":"10.1186/s13018-024-05414-3","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>We aimed to explore the mechanism by which Boron-doped nano-hydroxyapatite (B-nHAp) facilitates the proliferation and differentiation of osteoblasts through controlled release of B.</p><p><strong>Methods: </strong>B-nHAp characterization was accomplished by means of X-ray diffraction, scanning electron microscopy, inductively coupled plasma mass spectrometry, and transmission electron microscopy. Human bone marrow mesenchymal stem cells (hBMSCs) were subjected to flow cytometry, alizarin red S staining, and cell counting kit-8 assay for proliferation and differentiation determination. Western blotting for protein expression detection together with real-time quantitative polymerase chain reaction for mRNA expression measurement were carried out on those related to hBMSC proliferation and differentiation. Immunofluorescence staining was conducted to determine the activity of the Wnt/β-catenin signaling pathway.</p><p><strong>Results: </strong>B-nHAp particles had structured configuration and uniform size, and a typical nHAp crystal structure. The B content in B-nHAp was in line with expectation. hBMSCs displayed stemness. B-nHAp significantly facilitated the proliferation of hBMSCs, and significantly more mineralized nodules formed in the B-nHAp group. B-nHAp significantly upregulated the expressions of marker molecules related to hBMSC proliferation and differentiation. B-nHAp boosted the activity of the Wnt/β-catenin signaling pathway.</p><p><strong>Conclusion: </strong>B-nHAp modulates the Wnt/β-catenin signaling pathway to significantly enhance the proliferative and differential abilities of osteoblasts, potentially as an efficient material for bone repair.</p>","PeriodicalId":16629,"journal":{"name":"Journal of Orthopaedic Surgery and Research","volume":"20 1","pages":"62"},"PeriodicalIF":2.8000,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11748584/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Orthopaedic Surgery and Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s13018-024-05414-3","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ORTHOPEDICS","Score":null,"Total":0}
引用次数: 0
Abstract
Purpose: We aimed to explore the mechanism by which Boron-doped nano-hydroxyapatite (B-nHAp) facilitates the proliferation and differentiation of osteoblasts through controlled release of B.
Methods: B-nHAp characterization was accomplished by means of X-ray diffraction, scanning electron microscopy, inductively coupled plasma mass spectrometry, and transmission electron microscopy. Human bone marrow mesenchymal stem cells (hBMSCs) were subjected to flow cytometry, alizarin red S staining, and cell counting kit-8 assay for proliferation and differentiation determination. Western blotting for protein expression detection together with real-time quantitative polymerase chain reaction for mRNA expression measurement were carried out on those related to hBMSC proliferation and differentiation. Immunofluorescence staining was conducted to determine the activity of the Wnt/β-catenin signaling pathway.
Results: B-nHAp particles had structured configuration and uniform size, and a typical nHAp crystal structure. The B content in B-nHAp was in line with expectation. hBMSCs displayed stemness. B-nHAp significantly facilitated the proliferation of hBMSCs, and significantly more mineralized nodules formed in the B-nHAp group. B-nHAp significantly upregulated the expressions of marker molecules related to hBMSC proliferation and differentiation. B-nHAp boosted the activity of the Wnt/β-catenin signaling pathway.
Conclusion: B-nHAp modulates the Wnt/β-catenin signaling pathway to significantly enhance the proliferative and differential abilities of osteoblasts, potentially as an efficient material for bone repair.
期刊介绍：
Journal of Orthopaedic Surgery and Research is an open access journal that encompasses all aspects of clinical and basic research studies related to musculoskeletal issues.
Orthopaedic research is conducted at clinical and basic science levels. With the advancement of new technologies and the increasing expectation and demand from doctors and patients, we are witnessing an enormous growth in clinical orthopaedic research, particularly in the fields of traumatology, spinal surgery, joint replacement, sports medicine, musculoskeletal tumour management, hand microsurgery, foot and ankle surgery, paediatric orthopaedic, and orthopaedic rehabilitation. The involvement of basic science ranges from molecular, cellular, structural and functional perspectives to tissue engineering, gait analysis, automation and robotic surgery. Implant and biomaterial designs are new disciplines that complement clinical applications.
JOSR encourages the publication of multidisciplinary research with collaboration amongst clinicians and scientists from different disciplines, which will be the trend in the coming decades.
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