LINC00941 affects the proliferation, apoptosis and differentiation of osteoblasts by regulating the miR-335-5p/KAT7 axis.

IF 2.8 3区医学 Q1 ORTHOPEDICS

Journal of Orthopaedic Surgery and Research Pub Date : 2025-01-21 DOI:10.1186/s13018-025-05469-w

Longjin Liu, Ye Yang, Pengxiao Sun

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引用次数: 0

Abstract

Background: Fractures are the prevalent traumatic conditions encountered in orthopedic practices. The rising incidence of fractures has emerged as a pressing global health concern. Although the majority of individuals with fractures experience complete recovery of bone structure and function, approximately 10% of those with fractures exhibit delayed fracture healing (DFH). The objective of this investigation was to explore the function and underlying mechanisms of LINC00941 in the advancement of DFH, as well as its involvement in the regulation of osteoblastic differentiation by regulating the miR-335-5p/KAT7 axis.

Methods: The expression levels of LINC00941, miR-335-5p, KAT7 and osteoblast differentiation-related markers were assessed using RT-qPCR. The proliferation of MC3T3-E1 cells was evaluated through the CCK-8 assay, and cell apoptosis was analyzed via flow cytometry. The targeted regulatory relationships between LINC00941 and miR-335-5p, as well as between miR-335-5p and KAT7 were verified by a dual-luciferase reporter gene assay.

Result: The expression of LINC00941 was significantly up regulated, while miR-335-5p exhibited a notable downregulation in DFH patients, both of LINC00941 and miR-335-5p have been identified as potential predicted markers for DFH. Furthermore, LINC00941 has been demonstrated to inhibit osteoblast proliferation, promote apoptosis, and suppress osteoblast differentiation through the regulation of the miR-335-5p/KAT7 axis.

Conclusion: LINC00941/ miR-335-5p/KAT7 axis may be a therapeutic target for DFH.

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LINC00941通过调控miR-335-5p/KAT7轴影响成骨细胞的增殖、凋亡和分化。

背景：骨折是骨科实践中常见的创伤性疾病。骨折发病率的上升已成为一个紧迫的全球健康问题。虽然大多数骨折患者的骨结构和功能完全恢复，但约10%的骨折患者表现为延迟骨折愈合（DFH）。本研究的目的是探讨LINC00941在DFH进展中的功能和潜在机制，以及它通过调节miR-335-5p/KAT7轴参与成骨细胞分化的调节。方法：采用RT-qPCR检测成骨细胞分化相关标志物LINC00941、miR-335-5p、KAT7的表达水平。CCK-8法检测MC3T3-E1细胞增殖情况，流式细胞术检测细胞凋亡情况。通过双荧光素酶报告基因实验验证了LINC00941与miR-335-5p、miR-335-5p与KAT7之间的靶向调控关系。结果：在DFH患者中，LINC00941的表达明显上调，而miR-335-5p的表达明显下调，LINC00941和miR-335-5p已被确定为DFH的潜在预测标志物。此外，LINC00941已被证明通过调控miR-335-5p/KAT7轴抑制成骨细胞增殖，促进细胞凋亡，抑制成骨细胞分化。结论：LINC00941/ miR-335-5p/KAT7轴可能是DFH的治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Orthopaedic Surgery and Research ORTHOPEDICS-

CiteScore

4.10

自引率

7.70%

发文量

494

审稿时长

>12 weeks

期刊介绍： Journal of Orthopaedic Surgery and Research is an open access journal that encompasses all aspects of clinical and basic research studies related to musculoskeletal issues. Orthopaedic research is conducted at clinical and basic science levels. With the advancement of new technologies and the increasing expectation and demand from doctors and patients, we are witnessing an enormous growth in clinical orthopaedic research, particularly in the fields of traumatology, spinal surgery, joint replacement, sports medicine, musculoskeletal tumour management, hand microsurgery, foot and ankle surgery, paediatric orthopaedic, and orthopaedic rehabilitation. The involvement of basic science ranges from molecular, cellular, structural and functional perspectives to tissue engineering, gait analysis, automation and robotic surgery. Implant and biomaterial designs are new disciplines that complement clinical applications. JOSR encourages the publication of multidisciplinary research with collaboration amongst clinicians and scientists from different disciplines, which will be the trend in the coming decades.