The Discovery of New Antibody in Autoimmune Disease Using a Novel Approach of Coombs Test Based on Flow Cytometry Method

IF 2.6 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Clinical Laboratory Analysis Pub Date : 2025-01-20 DOI:10.1002/jcla.25148

Anwar Ullah, Xuewei Ding, Xia Qi*, Hui Liu*

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引用次数: 0

Abstract

Background and Objective

The objective of this study was to investigate the antibody to RBC in autoimmune disease patients with ANA using sensitive Coombs test based on flow cytometry method.

Materials and Methods

Antinuclear antibodies (ANA) of autoimmune disease patients were added to red blood cells (RBCs) of blood group O. At the same time, healthy individuals' serums were also checked. The sample tubes were incubated for 30 min at 37°C. After incubation, each sample was analyzed on flow cytometry.

Results

The agglutination of antinuclear antibodies in autoimmune patients was observed, while in healthy people, there was no agglutination between RBCs and serum. A significant difference was found between the disease group and healthy group (p < 0.0001) showing that a statistical analysis was conducted to compare the presence of ANA agglutination between the two groups. The reported p-value of less than 0.0001 shows that the observed difference is highly significant. The serum stability test conducted over ten consecutive days demonstrated a CV of 6.90% in the test results, indicating favorable stability.

Conclusion

In conclusion, this study emphasizes the effectiveness of flow cytometry as a valuable tool for detecting RBC-bound antibodies and new antibodies in autoimmune disease patients. Its high sensitivity and accuracy have the potential to greatly improve diagnostic capabilities in clinical laboratories.

Abstract Image

查看原文本刊更多论文

利用基于流式细胞术的库姆斯试验新方法发现自身免疫性疾病的新抗体。

背景与目的：本研究的目的是利用基于流式细胞术的敏感Coombs试验研究自身免疫性疾病ANA患者的红细胞抗体。材料与方法：将自身免疫性疾病患者的抗核抗体（ANA）添加到o型血的红细胞中，同时对健康人的血清进行检测。样品管在37℃下孵育30 min。孵育后，用流式细胞术分析每个样品。结果：自身免疫性患者血清中存在抗核抗体的凝集，而健康人血清中无抗核抗体的凝集。结论：本研究强调了流式细胞术作为检测自身免疫性疾病患者红细胞结合抗体和新抗体的重要工具的有效性。它的高灵敏度和准确性有可能大大提高临床实验室的诊断能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Clinical Laboratory Analysis 医学-医学实验技术

CiteScore

5.60

自引率

7.40%

发文量

584

审稿时长

6-12 weeks

期刊介绍： Journal of Clinical Laboratory Analysis publishes original articles on newly developing modes of technology and laboratory assays, with emphasis on their application in current and future clinical laboratory testing. This includes reports from the following fields: immunochemistry and toxicology, hematology and hematopathology, immunopathology, molecular diagnostics, microbiology, genetic testing, immunohematology, and clinical chemistry.