Techniques and analytic workflow for spatial transcriptomics and its application to allergy and inflammation.

Abstract

Spatial profiling, through single-cell gene level expression data paired with cell localization, offers unprecedented biological insights within the intact spatial context of cells in healthy and diseased tissue, adding a novel dimension to data interpretation. This review summarizes recent developments in this field, its application to allergy and inflammation, and recent single-cell resolution platforms designed for spatial transcriptomics with a focus on data processing and analyses for efficient biological interpretation of data. By preserving spatial context, these technologies provide critical insights into tissue architecture and cellular interactions unattainable with traditional transcriptomics methods, such as revealing localized inflammatory cell network in atopic dermatitis, and T-cell interactions in the lung in chronic obstructive pulmonary disease. Spatial profiling offers opportunities for discovering novel biomarkers, defining compartmentalization of immune responses within tissues and individual diseases, and accelerating novel discoveries towards a greater understanding of fundamental disease mechanisms, and eventually towards the development of future targeted therapies.