Heparinized venous blood gas syringes are associated with a significant decrease in measured packed cell volume in dogs due to hemodilution.

IF 1.6 2区农林科学 Q2 VETERINARY SCIENCES

Javma-journal of The American Veterinary Medical Association Pub Date : 2025-01-17 DOI:10.2460/javma.24.11.0718

Coyne M Hopey, Dana Caldwell, Erin Binagia, Deborah A Keys, Tereza Stastny

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引用次数: 0

Abstract

Objective: To assess the effect of hemodilution on PCV when commercial preheparinized venous blood gas (PH-VBG) syringes are used compared to directly filled Hct tubes, and to evaluate the impact of delayed centrifugation on PCV.

Methods: A prospective study of 102 dogs presenting to a private emergency department between November 1, 2023, and February 29, 2024, with 306 corresponding blood samples. Samples were collected by direct venipuncture. Samples were analyzed with the use of 3 methods: directly filled microhematocrit tubes (direct), PH-VBG syringe (PH-VBG), and PH-VBG syringe with a 15-minute delay before centrifugation (delayed PH-VBG).

Results: Mean PCV of the direct method was 43.9%, the PH-VBG method was 41.7% (range, 11.0% to 60.0%; SD, 9.8%), and the delayed PH-VBG method was 40.1% (range, 7.0% to 60.0%; SD, 11.2%). When the direct and PH-VBG methods were compared, the direct method was biased on average 2.2% higher than the PH-VBG method, with limits of agreement (LOAs) of -0.9 to 5.3. The direct method was biased on average 3.8% higher than the delayed PH-VBG method, with LOAs of -4.3 to 11.9. In anemic dogs (n = 14), the direct method was biased on average 6.1% higher than the delayed PH-VBG method, with LOAs of -0.05 to 12.2. In nonanemic dogs (n = 88), the direct method was biased on average 2.3% higher than the PH-VBG method, with LOAs of -0.9 to 5.4.

Conclusions: Use of preheparinized syringes and delayed sample processing led to hemodilution and lower PCV values.

Clinical relevance: Direct venipuncture and immediate sample processing are recommended to ensure accurate PCV results.

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由于血液稀释，肝素化静脉血气注射器与狗的测量堆积细胞体积显着减少有关。

目的：比较使用市售预肝素化静脉血气（PH-VBG）注射器与直接填充Hct管时血液稀释对PCV的影响，并评价延迟离心对PCV的影响。方法：对2023年11月1日至2024年2月29日在一家私人急诊科就诊的102只狗进行前瞻性研究，采集306份相应的血液样本。采用直接静脉穿刺法采集标本。样品分析采用3种方法：直接填充微红细胞压积管（direct）、PH-VBG注射器（PH-VBG）和PH-VBG注射器，离心前延迟15分钟（延迟PH-VBG）。结果：直接法的平均PCV为43.9%，PH-VBG法的平均PCV为41.7%(范围11.0% ~ 60.0%；SD, 9.8%)，延迟PH-VBG法为40.1%(范围7.0% ~ 60.0%；SD, 11.2%)。当直接法和PH-VBG法进行比较时，直接法的偏差比PH-VBG法平均高2.2%，一致性限（LOAs）为-0.9 ~ 5.3。直接法的偏差比延迟PH-VBG法平均高3.8%，LOAs为-4.3 ~ 11.9。在贫血犬（n = 14）中，直接法的偏差比延迟PH-VBG法平均高6.1%，loa为-0.05 ~ 12.2。在非贫血犬（n = 88）中，直接法比PH-VBG法的偏差平均高2.3%，loa为-0.9 ~ 5.4。结论：使用预肝素化注射器和延迟样品处理导致血液稀释和PCV值降低。临床相关性：建议直接静脉穿刺和立即样品处理，以确保准确的PCV结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Javma-journal of The American Veterinary Medical Association 农林科学-兽医学

CiteScore

1.60

自引率

15.80%

发文量

539

审稿时长

6-16 weeks

期刊介绍： Published twice monthly, this peer-reviewed, general scientific journal provides reports of clinical research, feature articles and regular columns of interest to veterinarians in private and public practice. The News and Classified Ad sections are posted online 10 days to two weeks before they are delivered in print.