{"title":"Establishment of a mouse model of allergic asthma sensitized and triggered with PM2.5.","authors":"Shaohua Li, Wuzhuang Sun","doi":"10.1080/09603123.2025.2453054","DOIUrl":null,"url":null,"abstract":"<p><p>To establish a mouse model of asthma sensitized and challenged with PM2.5 extract, 48 female BALB/c mice were included in this analysis. They were divided into six groups: normal control, ovalbumin (OVA) control, three PM2.5 dose groups, and a PM2.5+OVA combined group. Mice received intraperitoneal injections of PBS, OVA, PM2.5, or OVA+PM2.5 every 7 days for three weeks, followed by a one-week intranasal challenge. Airway responsiveness to acetylcholine was measured 24 hours post-challenge. Lung and nasal tissues were analyzed for histopathology, and bronchoalveolar lavage fluid (BALF) was assessed for inflammatory cells and cytokines. Compared to controls, PM2.5 and PM2.5+OVA groups showed increased airway hyperresponsiveness, pathological changes, elevated serum IgE, and altered cytokine levels (higher IL-4, IL-13; lower IFN-γ). In conclusion, PM2.5 extract can successfully establish a mouse model of allergic asthma.</p>","PeriodicalId":14039,"journal":{"name":"International Journal of Environmental Health Research","volume":" ","pages":"1-12"},"PeriodicalIF":2.2000,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Environmental Health Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/09603123.2025.2453054","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
To establish a mouse model of asthma sensitized and challenged with PM2.5 extract, 48 female BALB/c mice were included in this analysis. They were divided into six groups: normal control, ovalbumin (OVA) control, three PM2.5 dose groups, and a PM2.5+OVA combined group. Mice received intraperitoneal injections of PBS, OVA, PM2.5, or OVA+PM2.5 every 7 days for three weeks, followed by a one-week intranasal challenge. Airway responsiveness to acetylcholine was measured 24 hours post-challenge. Lung and nasal tissues were analyzed for histopathology, and bronchoalveolar lavage fluid (BALF) was assessed for inflammatory cells and cytokines. Compared to controls, PM2.5 and PM2.5+OVA groups showed increased airway hyperresponsiveness, pathological changes, elevated serum IgE, and altered cytokine levels (higher IL-4, IL-13; lower IFN-γ). In conclusion, PM2.5 extract can successfully establish a mouse model of allergic asthma.
期刊介绍：
International Journal of Environmental Health Research ( IJEHR ) is devoted to the rapid publication of research in environmental health, acting as a link between the diverse research communities and practitioners in environmental health. Published articles encompass original research papers, technical notes and review articles. IJEHR publishes articles on all aspects of the interaction between the environment and human health. This interaction can broadly be divided into three areas: the natural environment and health – health implications and monitoring of air, water and soil pollutants and pollution and health improvements and air, water and soil quality standards; the built environment and health – occupational health and safety, exposure limits, monitoring and control of pollutants in the workplace, and standards of health; and communicable diseases – disease spread, control and prevention, food hygiene and control, and health aspects of rodents and insects. IJEHR is published in association with the International Federation of Environmental Health and includes news from the Federation of international meetings, courses and environmental health issues.
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