The relationship between GABA content and desiccation tolerance at five developmental stages of wheat (Triticum durum) seeds.

IF 2.6 4区 生物学 Q2 PLANT SCIENCES
Nezar H Samarah, Nisreen A Al-Quraan, Roa'a I Shawah'en
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引用次数: 0

Abstract

Drying wheat (Triticum durum ) seeds within their spikes may improve the seed desiccation tolerance. This study aimed to understand the effect of drying wheat seeds within their spikes on their desiccation tolerance in association with GABA (γ-aminobutyric acid) content, malondialdehyde (MDA), the expression of three dehydrin genes (dhn , wcor , dreb ) during seed development. Seeds of wheat variety 'Hourani-Nawawi' were harvested at five developmental stages: (1) milk (ML); (2) soft dough (SD); (3) hard dough (HD); (4) physiological maturity (PM); and (5) harvest maturity (HM) and dried either attached to or detached from their spikes. Drying the seeds attached to their spikes improved desiccation tolerance, speed of germination, and seedling length at ML stage. Before drying (freshly harvested), the seeds harvested at ML and HM had higher GABA than those at SD, HD, and PM. The attached-dried seeds had higher GABA content from ML to PM than at HM, and higher glutamate content at ML, SD, and HD than at the PM stage. Detached-dried seeds had the highest alanine at ML and PM. Attached-dried seeds had lower MDA than detached-dried seeds. Expression of dhn was highest in freshly-harvested and attached-dried seeds at SD. Highest expression of wcor in the attached-dried seeds was detected at SD and HM. Drying the seeds within their spikes increased the expression of dreb gene compared with the freshly-harvested seeds, except at the HD stage. In conclusion, drying the seeds within their spikes enhanced seed germination in association with higher GABA, lower MDA, and higher gene expression.

小麦种子5个发育阶段GABA含量与脱水耐性的关系
小麦(Triticum durum)种子在穗内干燥可以提高种子的耐干燥性。本研究旨在了解小麦种子穗内干燥与种子发育过程中GABA (γ-氨基丁酸)含量、丙二醛(MDA)含量以及3种脱氢基因(dhn、wcor、dreb)表达的关系。小麦品种‘Hourani-Nawawi’的种子在五个发育阶段收获:(1)牛奶(ML);(2)软面团(SD);(3)硬面团(HD);(4)生理成熟度(PM);(5)收获成熟(HM)和干燥,附着或从穗上分离。将附着在穗状花序上的种子干燥后,可提高植株的耐干燥性、萌发速度和幼苗长度。干燥前(新鲜收获),ML和HM收获的种子GABA含量高于SD、HD和PM收获的种子。贴附干种子在ML - PM阶段的GABA含量高于HM阶段,在ML、SD和HD阶段的谷氨酸含量高于PM阶段。分离干种子在ML和PM时丙氨酸含量最高。贴附干种子的丙二醛含量低于分离干种子。在SD时,新鲜收获和贴附的干燥种子中dhn的表达量最高。wcor在贴附干种子中以SD和HM表达量最高。与新鲜收获的种子相比,在穗内干燥的种子增加了dreb基因的表达,但在HD阶段除外。综上所述,在种子穗内干燥可以提高GABA含量、降低MDA含量和提高基因表达,从而促进种子萌发。
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来源期刊
Functional Plant Biology
Functional Plant Biology 生物-植物科学
CiteScore
5.50
自引率
3.30%
发文量
156
审稿时长
1 months
期刊介绍: Functional Plant Biology (formerly known as Australian Journal of Plant Physiology) publishes papers of a broad interest that advance our knowledge on mechanisms by which plants operate and interact with environment. Of specific interest are mechanisms and signal transduction pathways by which plants adapt to extreme environmental conditions such as high and low temperatures, drought, flooding, salinity, pathogens, and other major abiotic and biotic stress factors. FPB also encourages papers on emerging concepts and new tools in plant biology, and studies on the following functional areas encompassing work from the molecular through whole plant to community scale. FPB does not publish merely phenomenological observations or findings of merely applied significance. Functional Plant Biology is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science. Functional Plant Biology is published in affiliation with the Federation of European Societies of Plant Biology and in Australia, is associated with the Australian Society of Plant Scientists and the New Zealand Society of Plant Biologists.
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