Detection of respiratory frequency rhythm in human alpha phase shifts: topographic distributions in wake and drowsy states.

Abstract

Introduction: The relationship between brain activity and respiration is recently attracting increasing attention, despite being studied for a long time. Respiratory modulation was evidenced in both single-cell activity and field potentials. Among EEG and intracranial measurements, the effect of respiration was prevailingly studied on amplitude/power in all frequency bands.

Methods: Since phases of EEG oscillations received less attention, we applied our previously published carrier frequency (CF) mathematical model of human alpha oscillations on a group of 10 young healthy participants in wake and drowsy states, using a 14-channel average reference montage. Since our approach allows for a more precise calculation of CF phase shifts (CFPS) than any individual Fourier component, by using a 2-s moving Fourier window, we validated the new method and studied, for the first time, temporal waveforms CFPS(t) and their oscillatory content through FFT (CFPS(t)).

Results: Although not appearing equally in all channel pairs and every subject, a clear peak in the respiratory frequency region, 0.21-0.26 Hz, was observed (max at 0.22 Hz). When five channel pairs with the most prominent group averaged amplitudes at 0.22 Hz were plotted in both states, topographic distributions changed significantly-from longitudinal, connecting frontal and posterior channels in the wake state to topographically split two separate regions-frontal and posterior in the drowsy state. In addition, in the drowsy state, 0.22-Hz amplitudes decreased for all pairs, while statistically significant reduction was obtained for 20/91 (22%) pairs.

Discussion: These results potentially evidence, for the first time, the respiratory frequency modulation of alpha phase shifts, as well as the significant impact of wakeful consciousness on the observed oscillations.