Exploring apoptotic pathways in SH-SY5Y neuroblastoma cells: combined effects of napabucasin and doxorubicin.

IF 1.2 4区医学 Q3 ANATOMY & MORPHOLOGY

Folia morphologica Pub Date : 2025-01-17 DOI:10.5603/fm.103887

Ilker Ünlü, Mehmet Cudi Tuncer, Ilhan Özdemir

{"title":"Exploring apoptotic pathways in SH-SY5Y neuroblastoma cells: combined effects of napabucasin and doxorubicin.","authors":"Ilker Ünlü, Mehmet Cudi Tuncer, Ilhan Özdemir","doi":"10.5603/fm.103887","DOIUrl":null,"url":null,"abstract":"Background: Neuroblastoma often begins in infancy and one of the most common types of cancer among children is someone. Napabucasin (NP) (BBI608), a natural naphthoquinone emerging as a novel inhibitor of STAT3, has been found to effectively kill cancer stem-like tumor cells. On the other hand, the effect of Napabucasin on SH-SY5Y cells is currently unclear. The effects and mechanisms of NP and doxorubicin (DX) on human metastatic neuroblastoma cells were investigated.Materials and methods: In this study, human neuroblastoma cells line (SHSY-5Y) were used. Apoptotic activation of NP and DX via the Bcl-2/Bax signaling pathway was evaluated by qRT-PCR, western blot and Tali cytometry. It was also detected by MTT, a cell viability test.Results: NP and DX antiproliferative and invasive effected to SH-SY5Y cells. Additionally, NP induced apoptosis by pausing the cell cycle. Moreover, NP treatment inhibited the expression of Bcl-2, which is associated with apoptosis, while it clearly inhibited the expression of Bax and CASP3 genes.Conclusions: As a results showed that NP and DX suppressed the proliferation of neuroblastoma cells and could do this through apoptotic pathways. NP can be used to suppress metastasis of SHSY-5Y cells as an inhibitor of the apoptosis pathway Bcl-2. It is thought that NP, which provides tumor suppression through an apoptotic mechanism, may be an alternative treatment agent in neurological cancers such as neuroblastoma.","PeriodicalId":12251,"journal":{"name":"Folia morphologica","volume":" ","pages":""},"PeriodicalIF":1.2000,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Folia morphologica","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.5603/fm.103887","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ANATOMY & MORPHOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Neuroblastoma often begins in infancy and one of the most common types of cancer among children is someone. Napabucasin (NP) (BBI608), a natural naphthoquinone emerging as a novel inhibitor of STAT3, has been found to effectively kill cancer stem-like tumor cells. On the other hand, the effect of Napabucasin on SH-SY5Y cells is currently unclear. The effects and mechanisms of NP and doxorubicin (DX) on human metastatic neuroblastoma cells were investigated.

Materials and methods: In this study, human neuroblastoma cells line (SHSY-5Y) were used. Apoptotic activation of NP and DX via the Bcl-2/Bax signaling pathway was evaluated by qRT-PCR, western blot and Tali cytometry. It was also detected by MTT, a cell viability test.

Results: NP and DX antiproliferative and invasive effected to SH-SY5Y cells. Additionally, NP induced apoptosis by pausing the cell cycle. Moreover, NP treatment inhibited the expression of Bcl-2, which is associated with apoptosis, while it clearly inhibited the expression of Bax and CASP3 genes.

Conclusions: As a results showed that NP and DX suppressed the proliferation of neuroblastoma cells and could do this through apoptotic pathways. NP can be used to suppress metastasis of SHSY-5Y cells as an inhibitor of the apoptosis pathway Bcl-2. It is thought that NP, which provides tumor suppression through an apoptotic mechanism, may be an alternative treatment agent in neurological cancers such as neuroblastoma.

查看原文本刊更多论文

探索SH-SY5Y神经母细胞瘤细胞的凋亡途径：纳布卡霉素和阿霉素的联合作用。

背景：神经母细胞瘤通常开始于婴儿期，儿童中最常见的癌症类型之一是某人。Napabucasin (NP) （BBI608）是一种天然萘醌，是一种新型的STAT3抑制剂，已被发现可有效杀死肿瘤干细胞样肿瘤细胞。另一方面，Napabucasin对SH-SY5Y细胞的作用目前尚不清楚。研究了NP和多柔比星（DX）对人转移性神经母细胞瘤细胞的作用及其机制。材料与方法：本研究采用人神经母细胞瘤细胞系SHSY-5Y。采用qRT-PCR、western blot和Tali细胞术检测Bcl-2/Bax信号通路对NP和DX的凋亡激活作用。用细胞活力试验MTT检测。结果：NP和DX对SH-SY5Y细胞有抗增殖和侵袭作用。此外，NP通过暂停细胞周期诱导细胞凋亡。此外，NP处理抑制了与凋亡相关的Bcl-2的表达，同时明显抑制了Bax和CASP3基因的表达。结论：NP和DX对神经母细胞瘤细胞增殖的抑制作用可能通过凋亡途径实现。NP可作为凋亡通路Bcl-2的抑制剂抑制SHSY-5Y细胞的转移。NP通过细胞凋亡机制抑制肿瘤，可能成为神经系统肿瘤（如神经母细胞瘤）的替代治疗药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Folia morphologica ANATOMY & MORPHOLOGY-

CiteScore

2.40

自引率

0.00%

发文量

218

审稿时长

6-12 weeks

期刊介绍： "Folia Morphologica" is an official journal of the Polish Anatomical Society (a Constituent Member of European Federation for Experimental Morphology - EFEM). It contains original articles and reviews on morphology in the broadest sense (descriptive, experimental, and methodological). Papers dealing with practical application of morphological research to clinical problems may also be considered. Full-length papers as well as short research notes can be submitted. Descriptive papers dealing with non-mammals, cannot be accepted for publication with some exception.