SMILE-Derived Corneal Stromal Lenticule: Experimental Study as a Corneal Repair Material and Drug Carrier.

IF 1.9 3区医学 Q2 OPHTHALMOLOGY

Cornea Pub Date : 2025-01-21 DOI:10.1097/ICO.0000000000003787

Qin Wang, Jing Rao, Min Zhang, Ling Zhou, Xiao Chen, Yanran Ma, Hongjian Guo, Jianing Gu, Yini Wang, Qizhi Zhou

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Abstract

Purpose: A detailed study of the physicochemical properties of SMILE-derived lenticules and evaluation of their drug delivery after loading with silver nanoparticles (AgNPs).

Methods: The lenticules were decellularized and modified with crosslinking concentrations of 0.01 (0.01E/L), 0.05 (0.05E/L), and 0.25 (0.25E/L) mmol N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) per mg lenticule at 5:1 carbodiimide/N-hydroxysuccinimide (EDC/NHS) ratios. The degree of swelling, light transmittance, biomechanical properties, and stability of the non-crosslinked decellularized lenticules (DLs), 0.01E/L, 0.05E/L, and 0.25E/L were measured and characterized using Fourier transform infrared spectroscopy and transmission electron microscopy with non-crosslinked non-decellularized lenticules as controls. DLs, 0.01E/L, 0.05E/L, and 0.25E/L were soaked in AgNPs for 24 hours, and the concentration of the drug released was measured.

Results: There was no significant difference in the degree of swelling between the groups (P > 0.05). The light transmittance of the lenticules did not change after decellularization and crosslinking and decreased after loading with AgNPs. Non-decellularized lenticules biodegraded within 108 to 120 hours, and the other groups biodegraded within 96 to 108 hours in vitro. The 0.01E/L had the highest tensile strength. The absorption peak intensity ratio of the amide I band and the amide II band decreased, and the arrangement of collagen fibers was more compact in crosslinked decellularized lenticules. The 0.01E/L had the highest cumulative drug release (3.4 ± 0.91 μg).

Conclusions: Crosslinking decellularization improved the biomechanical properties and resistance to water absorption of lenticules, increased covalent bonds between collagen fibers, and improved drug delivery. Crosslinked decellularized lenticules can be used as a new corneal patch material and drug delivery carrier for drug AgNPs.

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smile衍生角膜基质透镜：作为角膜修复材料和药物载体的实验研究。

目的：详细研究smile衍生透镜体的物理化学性质，并评价其载银纳米颗粒（AgNPs）后的给药性能。方法：采用交联浓度分别为0.01 （0.01 e/ L）、0.05 （0.05 e/ L）、0.25 (0.25 e/ L) mmol N-（3-二甲氨基丙基）-N′-乙基碳二亚胺(EDC) / mg / mg，碳二亚胺/N-羟基琥珀酰亚胺（EDC/NHS）比为5:1的方法进行脱细胞修饰。以非交联非脱细胞透镜体为对照，采用傅里叶变换红外光谱和透射电镜对非交联非脱细胞透镜体（dl）、0.01E/L、0.05E/L和0.25E/L的溶胀度、透光率、生物力学性能和稳定性进行了测量和表征。将DLs、0.01E/L、0.05E/L、0.25E/L分别浸泡于AgNPs中24h，测定药物释放浓度。结果：各组大鼠肿胀程度差异无统计学意义（P < 0.05）。在脱细胞和交联后，微孔的透光率没有变化，而加载AgNPs后透光率下降。未脱细胞的透镜体在108至120小时内生物降解，其他组在96至108小时内生物降解。0.01E/L的拉伸强度最高。酰胺I带和酰胺II带的吸收峰强度比减小，胶原纤维在交联脱细胞小泡中的排列更加紧密。0.01E/L的累积释药量最高（3.4±0.91 μg）。结论：交联脱细胞改善了皮囊的生物力学性能和抗吸水性能，增加了胶原纤维之间的共价键，改善了药物传递。交联脱细胞小体可作为一种新型角膜贴片材料和药物AgNPs的药物传递载体。

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来源期刊

Cornea 医学-眼科学

CiteScore

5.20

自引率

10.70%

发文量

354

审稿时长

3-6 weeks

期刊介绍： For corneal specialists and for all general ophthalmologists with an interest in this exciting subspecialty, Cornea brings together the latest clinical and basic research on the cornea and the anterior segment of the eye. Each volume is peer-reviewed by Cornea''s board of world-renowned experts and fully indexed in archival format. Your subscription brings you the latest developments in your field and a growing library of valuable professional references. Sponsored by The Cornea Society which was founded as the Castroviejo Cornea Society in 1975.