A structural view of nickel-pincer nucleotide cofactor-related biochemistry.

IF 6.2 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Critical Reviews in Biochemistry and Molecular Biology Pub Date : 2025-01-19 DOI:10.1080/10409238.2025.2451443

Santhosh Gatreddi, Shramana Chatterjee, Aiko Turmo, Jian Hu, Robert P Hausinger

引用次数: 0

Abstract

The nickel-pincer nucleotide (NPN) is an organometallic cofactor that was first discovered in lactate racemase from Lactiplantibacillus plantarum. In this review, we provide an overview on the structure-function relationships of enzymes that utilize or are involved in the biosynthesis of the NPN cofactor. Recent structural advances have greatly extended our understanding of the biological role of the NPN cofactor in a diverse family of 2-hydroxyacid racemases and epimerases. Moreover, structural studies of the accessory proteins LarB (a combined carboxylase/hydrolase), two distinct forms of LarE (an ATP-dependent sulfur transferase), and LarC (a CTP-dependent nickel insertase) have elucidated key features in the biosynthetic pathway for the NPN cofactor. Finally, we discuss the potential of future structural investigations to uncover additional enzymes that synthesize and use the NPN cofactor to catalyze new reactions.

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钳镍核苷酸辅因子相关生物化学的结构观点。

镍钳核苷酸（NPN）是一种有机金属辅助因子，最早发现于植物乳杆菌的乳酸消旋酶中。本文综述了利用或参与NPN辅助因子生物合成的酶的结构-功能关系。最近的结构进展极大地扩展了我们对NPN辅助因子在2-羟基酸外消旋酶和外缩酶不同家族中的生物学作用的理解。此外，对辅助蛋白LarB（一种组合羧化酶/水解酶）、两种不同形式的LarE（一种依赖atp的硫转移酶）和LarC（一种依赖ctp的镍插入酶）的结构研究已经阐明了NPN辅助因子生物合成途径的关键特征。最后，我们讨论了未来结构研究的潜力，以发现合成和使用NPN辅助因子催化新反应的其他酶。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Critical Reviews in Biochemistry and Molecular Biology 生物-生化与分子生物学

CiteScore

14.90

自引率

0.00%

发文量

期刊介绍： As the discipline of biochemistry and molecular biology have greatly advanced in the last quarter century, significant contributions have been made towards the advancement of general medicine, genetics, immunology, developmental biology, and biophysics. Investigators in a wide range of disciplines increasingly require an appreciation of the significance of current biochemical and molecular biology advances while, members of the biochemical and molecular biology community itself seek concise information on advances in areas remote from their own specialties. Critical Reviews in Biochemistry and Molecular Biology believes that well-written review articles prove an effective device for the integration and meaningful comprehension of vast, often contradictory, literature. Review articles also provide an opportunity for creative scholarship by synthesizing known facts, fruitful hypotheses, and new concepts. Accordingly, Critical Reviews in Biochemistry and Molecular Biology publishes high-quality reviews that organize, evaluate, and present the current status of high-impact, current issues in the area of biochemistry and molecular biology. Topics are selected on the advice of an advisory board of outstanding scientists, who also suggest authors of special competence. The topics chosen are sufficiently broad to interest a wide audience of readers, yet focused enough to be within the competence of a single author. Authors are chosen based on their activity in the field and their proven ability to produce a well-written publication.