MUSTN1 Interaction With SMPX Regulates Muscle Development and Regeneration.

IF 5.9 1区生物学 Q2 CELL BIOLOGY

Cell Proliferation Pub Date : 2025-01-19 DOI:10.1111/cpr.13809

Yu Fu, Xin Hao, Peng Shang, Jingru Nie, Yangzom Chamba, Bo Zhang, Hao Zhang

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Abstract

Pigs are important agricultural animals whose growth rate and meat production performance are related to muscle development. Musculoskeletal embryonic nuclear protein 1 (MUSTN1) participates in various biological processes, including myogenesis and growth in animals, but the physiological functions and mechanisms of porcine MUSTN1 on muscle development are unclear; thus, we aimed to elucidate them. We found that MUSTN1 was highly expressed in the muscles of fast-growing pigs. Functionally, MUSTN1 promoted myoblast proliferation and differentiation. MUSTN1 knockout mice exhibited reduced muscle mass and fibre cross-sectional area, decreased exercise endurance, and delayed muscle regeneration. Small muscle protein X-linked (SMPX) was identified as an interacting protein of MUSTN1, and its promotion of myogenic differentiation depended on MUSTN1. Furthermore, MUSTN1 stabilised SMPX and maintained myofiber morphology. This study suggests that MUSTN1 is a critical regulator in the control of muscle development and regeneration and is a potential target for animal genetic improvement and the treatment of human muscle disease.

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MUSTN1与SMPX的相互作用调节肌肉发育和再生。

猪是重要的农业动物，其生长速度和产肉性能与肌肉发育有关。肌骨骼胚胎核蛋白1 （MUSTN1）参与动物的多种生物过程，包括肌肉发生和生长，但猪MUSTN1在肌肉发育中的生理功能和机制尚不清楚；因此，我们的目的是阐明它们。我们发现MUSTN1在快速生长的猪的肌肉中高度表达。功能上，MUSTN1促进成肌细胞增殖和分化。MUSTN1基因敲除小鼠表现出肌肉质量和纤维横截面积减少，运动耐力下降，肌肉再生延迟。小肌蛋白X-linked （SMPX）被鉴定为MUSTN1的相互作用蛋白，其促进成肌分化依赖于MUSTN1。此外，MUSTN1稳定SMPX并维持肌纤维形态。这项研究表明，MUSTN1是控制肌肉发育和再生的关键调节因子，是动物遗传改良和治疗人类肌肉疾病的潜在靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Proliferation 生物-细胞生物学

CiteScore

14.80

自引率

2.40%

发文量

198

审稿时长

1 months

期刊介绍： Cell Proliferation Focus: Devoted to studies into all aspects of cell proliferation and differentiation. Covers normal and abnormal states. Explores control systems and mechanisms at various levels: inter- and intracellular, molecular, and genetic. Investigates modification by and interactions with chemical and physical agents. Includes mathematical modeling and the development of new techniques. Publication Content: Original research papers Invited review articles Book reviews Letters commenting on previously published papers and/or topics of general interest By organizing the information in this manner, readers can quickly grasp the scope, focus, and publication content of Cell Proliferation.