Assessment of Garbage Enzyme as a Bioremediation Method for the Wastewater Treatment.

IF 3.2 4区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Anoop Narain Singh, Maya Rajnarayan Ray, Umank Mishra, Kruti B Jethwa, Nishant Yadav, Nisha Shankhwar, Gulab Singh Chauhan, Kundan Meshram
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Abstract

This study evaluates the efficacy of garbage enzyme (GE) in bioremediation to reduce pollutants in sewage drains that discharge into the natural streams and rivers. Garbage enzyme is prepared with help of brown sugar, fruit, vegetable wastes, and water in the proportion 1:3:10 (by weight), which is then applied to the samples collected from various drainage sites in Jaunpur district, Uttar Pradesh, India. Different concentrations of GE (ranging from 0% to 20%) are mixed with sewage to assess pollution reduction. Different parameters, that is, pH, dissolved oxygen (DO), total suspended solids (TSS), total dissolved solids (TDS), biochemical oxygen demand (BOD), chemical oxygen demand (COD), and CD have been measured on 3rd, 5th, 7th, 10th, 15th, and 20th days. It has been observed in the study that significant percentage reduction in BOD, COD, TSS, and TDS of 80.6%, 62.9%, 74.29%, and 43.9%, respectively, while an increase in DO is 67.8% on 20th day of experiment with 20% GE addition. The result of the study reveals that there is highest reduction occurring on the 20th day with a 20% GE concentration. It has been observed that the GE possesses protease, amylase, and lipase activity. Meaning GE contains microorganisms that breakdown carbohydrates, fats, and proteins and it hydrolyzes these complex organic molecules into simpler compounds, making them more readily biodegradable and that is the reason for substantial reduction in the pollutants. Moreover, in this study it has been observed that there is a notable decrease in foul odor emanating from the samples. Time-dependent fluctuations in pH, TDS, DO, BOD, COD, and temperature are documented, with their correlations examined. This investigation underscores GE's potential in pollution mitigation, particularly in sewage systems, and offers valuable insights for sustainable environmental management practices aimed at conserving natural water bodies. Further tests are required to check the efficiency of treatment with GE dosage higher than 20%.

垃圾酶在污水处理中的生物修复效果评价。
本研究评估了垃圾酶(GE)在生物修复中减少排入自然溪流和河流的污水排放物中的污染物的效果。以红糖、水果、蔬菜废弃物和水按1:3:10(重量比)的比例制备垃圾酶,并将其应用于从印度北方邦Jaunpur地区各个排水点收集的样品。将不同浓度的GE(0%至20%)与污水混合,以评估污染减少情况。分别于第3、5、7、10、15、20天测定pH、溶解氧(DO)、总悬浮固体(TSS)、总溶解固体(TDS)、生化需氧量(BOD)、化学需氧量(COD)和CD等不同参数。研究发现,在试验第20天,添加20% GE后,BOD、COD、TSS和TDS分别降低了80.6%、62.9%、74.29%和43.9%,DO提高了67.8%。研究结果显示,当GE浓度为20%时,第20天的降幅最大。已经观察到GE具有蛋白酶、淀粉酶和脂肪酶活性。也就是说,GE含有分解碳水化合物、脂肪和蛋白质的微生物,并将这些复杂的有机分子水解成更简单的化合物,使它们更容易被生物降解,这就是污染物大幅减少的原因。此外,在这项研究中,它已经观察到,有一个显著的减少恶臭散发的样品。记录了pH、TDS、DO、BOD、COD和温度随时间变化的波动,并检验了它们之间的相关性。这项调查强调了通用电气在减少污染方面的潜力,特别是在污水处理系统方面,并为旨在保护自然水体的可持续环境管理实践提供了有价值的见解。需要进一步试验,以检查GE剂量高于20%的治疗效率。
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来源期刊
Biotechnology and applied biochemistry
Biotechnology and applied biochemistry 工程技术-生化与分子生物学
CiteScore
6.00
自引率
7.10%
发文量
117
审稿时长
3 months
期刊介绍: Published since 1979, Biotechnology and Applied Biochemistry is dedicated to the rapid publication of high quality, significant research at the interface between life sciences and their technological exploitation. The Editors will consider papers for publication based on their novelty and impact as well as their contribution to the advancement of medical biotechnology and industrial biotechnology, covering cutting-edge research in synthetic biology, systems biology, metabolic engineering, bioengineering, biomaterials, biosensing, and nano-biotechnology.
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