Out-of-focus signal rejection for in vivo pO2 measurements using two-photon phosphorescence lifetime microscopy.

IF 2.9 2区 医学 Q2 BIOCHEMICAL RESEARCH METHODS
Biomedical optics express Pub Date : 2024-12-12 eCollection Date: 2025-01-01 DOI:10.1364/BOE.532084
Qi Pian, Baoqiang Li, Ikbal Şencan-Eğilmez, Xiaojun Cheng, Jay Dubb, Xinyue Huang, Buyin Fu, Srinivasa Rao Allu, Mohammad Abbas Yaseen, Anna Devor, Sergei A Vinogradov, Sava Sakadžić
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引用次数: 0

Abstract

Two-photon phosphorescence lifetime microscopy has been a key tool for studying cerebral oxygenation in mice. However, the accuracy of the partial pressure of oxygen (pO2) measurements is affected by out-of-focus signal. In this work, we applied reconfigurable differential aberration imaging to characterize and correct for out-of-focus signal contamination in intravascular pO2 imaging. Our results show that signal contamination is higher in more oxygenated vessels and that it could be effectively removed using the proposed method.

用双光子磷光寿命显微镜测量体内pO2的失焦信号抑制。
双光子磷光寿命显微镜已成为研究小鼠脑氧合的重要工具。然而,失焦信号会影响氧分压(pO2)测量的准确性。在这项工作中,我们应用可重构差分像差成像来表征和纠正血管内pO2成像中的失焦信号污染。我们的研究结果表明,信号污染在氧气含量越高的血管中越高,并且可以使用所提出的方法有效地去除。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biomedical optics express
Biomedical optics express BIOCHEMICAL RESEARCH METHODS-OPTICS
CiteScore
6.80
自引率
11.80%
发文量
633
审稿时长
1 months
期刊介绍: The journal''s scope encompasses fundamental research, technology development, biomedical studies and clinical applications. BOEx focuses on the leading edge topics in the field, including: Tissue optics and spectroscopy Novel microscopies Optical coherence tomography Diffuse and fluorescence tomography Photoacoustic and multimodal imaging Molecular imaging and therapies Nanophotonic biosensing Optical biophysics/photobiology Microfluidic optical devices Vision research.
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