PTPA localized in the Golgi apparatus plays an important role in osteoblast differentiation

IF 2.5 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical and biophysical research communications Pub Date : 2025-01-13 DOI:10.1016/j.bbrc.2025.151329

Xinyu Zheng , Yao Weng , Ayano Satoh , Airi Tanai , Mika Ikegame , Koji Kimura , Nana Yoshitani , Xiaohua Xie , Hirohiko Okamura

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引用次数: 0

Abstract

Regulatory subunits of protein phosphatase 2A (PP2A) define the substrate and functional specificity of the PP2A holoenzyme within specific organelles. While PP2A regulates osteoblast differentiation, the roles and localization of its regulatory subunits in osteoblasts remain unclear. Here, we identified PTPA, a PP2A regulatory protein, predominantly localized to the Golgi apparatus, closely overlapping with the Golgi marker Giantin. Disruption of the Golgi structure by Brefeldin A caused PTPA to disperse into the cytoplasm. PTPA overexpression inhibited osteoblast differentiation by downregulating key transcriptional regulators. The Golgi-specific localization of PTPA suggests it may influence bone-related protein secretion and maintain Golgi integrity. These findings highlight the critical role of PTPA in osteoblast differentiation and its association with the Golgi apparatus.

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定位于高尔基体的PTPA在成骨细胞分化中起重要作用。

蛋白磷酸酶2A （PP2A）的调控亚基定义了PP2A全酶在特定细胞器内的底物和功能特异性。虽然PP2A调控成骨细胞分化，但其调控亚基在成骨细胞中的作用和定位尚不清楚。在这里，我们发现了PP2A调节蛋白PTPA，主要定位于高尔基体，与高尔基体标记物Giantin密切重叠。Brefeldin A破坏高尔基结构导致PTPA分散到细胞质中。PTPA过表达通过下调关键转录调控因子抑制成骨细胞分化。PTPA的高尔基特异性定位表明它可能影响骨相关蛋白的分泌并维持高尔基完整性。这些发现强调了PTPA在成骨细胞分化中的关键作用及其与高尔基体的关联。

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来源期刊

Biochemical and biophysical research communications 生物-生化与分子生物学

CiteScore

6.10

自引率

0.00%

发文量

1400

审稿时长

14 days

期刊介绍： Biochemical and Biophysical Research Communications is the premier international journal devoted to the very rapid dissemination of timely and significant experimental results in diverse fields of biological research. The development of the "Breakthroughs and Views" section brings the minireview format to the journal, and issues often contain collections of special interest manuscripts. BBRC is published weekly (52 issues/year).Research Areas now include: Biochemistry; biophysics; cell biology; developmental biology; immunology ; molecular biology; neurobiology; plant biology and proteomics