Labelling of a live obligate anaerobe using fluorescent D-amino acids.

IF 2.5 3区生物学 Q3 MICROBIOLOGY

Anaerobe Pub Date : 2025-01-13 DOI:10.1016/j.anaerobe.2025.102939

Benjamin Lewis, Adenrele Oludiran, Ann Progulske-Fox, William Dunn

引用次数: 0

Abstract

The probing of live bacteria via the incorporation of fluorescent D-amino acids (FDAAs) during peptidoglycan synthesis has been shown to be practical for visualizing both gram-positive and gram-negative bacterial species. This study demonstrates the reliability and applications of FDAA labelling for the fluorescent imaging of an obligate anaerobe.

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用荧光d -氨基酸标记活的专性厌氧菌。

在肽聚糖合成过程中，通过荧光d -氨基酸（FDAAs）的掺入来探测活菌，已被证明对革兰氏阳性和革兰氏阴性细菌物种的可视化都是可行的。本研究证明了FDAA标记在专性厌氧菌荧光成像中的可靠性和应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Anaerobe 生物-微生物学

CiteScore

5.20

自引率

8.70%

发文量

137

审稿时长

76 days

期刊介绍： Anaerobe is essential reading for those who wish to remain at the forefront of discoveries relating to life processes of strictly anaerobes. The journal is multi-disciplinary, and provides a unique forum for those investigating anaerobic organisms that cause infections in humans and animals, as well as anaerobes that play roles in microbiomes or environmental processes. Anaerobe publishes reviews, mini reviews, original research articles, notes and case reports. Relevant topics fall into the broad categories of anaerobes in human and animal diseases, anaerobes in the microbiome, anaerobes in the environment, diagnosis of anaerobes in clinical microbiology laboratories, molecular biology, genetics, pathogenesis, toxins and antibiotic susceptibility of anaerobic bacteria.