Antibacterial Activity of Crude Momordica charantia, Cassia alata, and Allium sativum Methanolic Extracts on Leptospira interrogans serovar Manilae.

Q4 Medicine
Acta Medica Philippina Pub Date : 2024-12-13 eCollection Date: 2024-01-01 DOI:10.47895/amp.vi0.8362
Fatima Ericka S Vista, Bryan Paul D De Galicia
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引用次数: 0

Abstract

Background and objective: Leptospirosis is a disease caused by pathogenic Leptospira prevalent in tropical countries like the Philippines. Some studies have shown that the role of currently used antibiotics for leptospirosis is unclear since trials have found no significant benefit to patient outcomes compared to placebo. This signals the need for alternative therapies, such as herbal medicines, which may provide effective therapeutic regimens in treating this infection. In this study, we characterized the antibacterial potential of three Philippine herbal medicines against Leptospira interrogans.

Methods: Crude methanolic extracts of Momordica charantia, Cassia alata, and Allium sativum were subjected to an optimized broth microdilution assay against L. interrogans, utilizing the resazurin-resorufin reaction as a cell proliferation and viability indicator.

Results: The respective minimum inhibitory concentrations of the plants were found to be as follows: 1.25 mg/mL (M. charantia), 2.5 mg/mL (C. alata), and >5 mg/mL (A. sativum).

Conclusions: Among the three herbal medicines, M. charantia and C. alata proved to have antibacterial activity against L. interrogans. Given the promising potential of two of these plant extracts, exploring the use of other solvents to extract natural compounds from these plants, and discovering possible synergistic effects between these plants and conventional antibiotics may be worthwhile.

苦瓜、决明子和葱醇提取物对马尼拉钩端螺旋体的抑菌活性。
背景和目的:钩端螺旋体病是一种由致病性钩端螺旋体引起的疾病,流行于菲律宾等热带国家。一些研究表明,目前使用的抗生素治疗钩端螺旋体病的作用尚不清楚,因为试验发现与安慰剂相比,对患者的预后没有显著的益处。这表明需要替代疗法,如草药,这可能为治疗这种感染提供有效的治疗方案。在这项研究中,我们表征了三种菲律宾草药对钩端螺旋体的抗菌潜力。方法:采用优化后的肉汤微量稀释法,对苦瓜、清明和葱的粗甲醇提取物进行抑菌试验,以瑞祖脲-间苯二酚反应为细胞增殖和活力指标。结果:三种植物的最低抑菌浓度分别为1.25 mg/mL、2.5 mg/mL和5 mg/mL。结论:在3种中草药中,沙兰草和苍耳草具有一定的抑菌活性。鉴于这两种植物提取物的潜力,探索使用其他溶剂从这些植物中提取天然化合物,并发现这些植物与传统抗生素之间可能的协同作用可能是值得的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Acta Medica Philippina
Acta Medica Philippina Medicine-Medicine (all)
CiteScore
0.40
自引率
0.00%
发文量
199
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