p16INK4a / Ki67 dual staining immunocytochemistry to refer women infected by HR-HPV for colposcopy.

IF 1.6 4区医学 Q3 PATHOLOGY

Acta Cytologica Pub Date : 2025-01-17 DOI:10.1159/000542504

Magnus von Knebel Doeberitz

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引用次数: 0

Abstract

Background: Cervical cancer is still a significant global health issue, espacially in low- and middle-income countries. Human papillomavirus (HPV) infection is known to be the primary etiological factor in the development of cervical cancer. The identification of high-risk HPV infections in cervical screening is becoming crucial for early detection and management of cervical pre-neoplasia. However, HPV testing requires triage markers since HR HPV infections are widespread in the target population, of which only a small fraction requires detailed clinical work-up by colposcopy.

Summary: p16INK4a is a tumor suppressor protein that has emerged as a promising surrogate marker for HPV infection. In combination with a proliferation marker Ki67 in one diagnostic test kit referred to as CINtecPlus®- or dual stain (DS) it revealed excellent sensitivity and specificity profiles to detect clinically relevant CIN2+ lesions among HPV-infected women in large screening cohorts.

Key message: This review examines the role of the cytology CINtecPlus®-test (Dual Stain (DS)-test) as a triage marker to identify patients with CIN2+ lesions among HPV + tested women and summarizes the most recent recommendations for its clinical use.

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p16INK4a / Ki67双染色免疫细胞化学对HR-HPV感染妇女阴道镜检查的参考价值。

背景：子宫颈癌仍然是一个重大的全球健康问题，特别是在低收入和中等收入国家。人乳头瘤病毒（HPV）感染被认为是宫颈癌发展的主要病因。宫颈筛查中高危HPV感染的识别对于早期发现和管理宫颈癌前病变至关重要。然而，HPV检测需要分诊标记，因为HR HPV感染在目标人群中广泛存在，其中只有一小部分需要通过阴道镜进行详细的临床检查。摘要：p16INK4a是一种肿瘤抑制蛋白，已成为HPV感染的替代标志物。在一种被称为CINtecPlus®或双染色（DS）的诊断测试试剂盒中，与增殖标志物Ki67联合使用，在大型筛查队列中检测hpv感染女性临床相关的CIN2+病变时，显示出极好的敏感性和特异性。关键信息：本综述探讨了细胞学CINtecPlus®测试（双染色（DS）测试）作为鉴别HPV +检测女性中CIN2+病变患者的分诊标记的作用，并总结了其临床应用的最新建议。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Cytologica 生物-病理学

CiteScore

3.70

自引率

11.10%

发文量

审稿时长

4-8 weeks

期刊介绍： With articles offering an excellent balance between clinical cytology and cytopathology, ''Acta Cytologica'' fosters the understanding of the pathogenetic mechanisms behind cytomorphology and thus facilitates the translation of frontline research into clinical practice. As the official journal of the International Academy of Cytology and affiliated to over 50 national cytology societies around the world, ''Acta Cytologica'' evaluates new and existing diagnostic applications of scientific advances as well as their clinical correlations. Original papers, review articles, meta-analyses, novel insights from clinical practice, and letters to the editor cover topics from diagnostic cytopathology, gynecologic and non-gynecologic cytopathology to fine needle aspiration, molecular techniques and their diagnostic applications. As the perfect reference for practical use, ''Acta Cytologica'' addresses a multidisciplinary audience practicing clinical cytopathology, cell biology, oncology, interventional radiology, otorhinolaryngology, gastroenterology, urology, pulmonology and preventive medicine.