Artificial cell-free system for the sustainable production of acetoin from bioethanol.

IF 9.7 1区 环境科学与生态学 Q1 AGRICULTURAL ENGINEERING
David Muñoz-Sánchez, Albert Carceller, Gregorio Álvaro, Óscar Romero, Marina Guillén
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引用次数: 0

Abstract

The present work introduces and validates an artificial cell free system for the synthesis of acetoin from ethanol, representing a greener alternative to conventional chemical synthesis. The one pot multi-enzymatic system, which employs pyruvate decarboxylase from Zymobacter palmae (ZpPDC), alcohol dehydrogenase from Saccharomyces cerevisiae (ScADH), and NADH oxidase from Streptococcus pyogenes (SpNOX), achieves nearly 100 % substrate conversion and reaction yield within 6 h under optimal conditions (pH 7.5, enzyme activities: ZpPDC 100 U·mL-1, ScADH 50 U·mL-1, SpNOX 127 U·mL-1, and 1 mM NAD+). Using air for oxygen supply mitigates enzyme inactivation while effectively accelerating the regeneration of NAD+. The use of bioethanol as a substrate demonstrates the robustness and sustainability of the bioprocess, enabling the production of natural acetoin from renewable resources. This environmentally friendly approach offers significant advantages for industrial applications, aligning with green chemistry principles.

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来源期刊
Bioresource Technology
Bioresource Technology 工程技术-能源与燃料
CiteScore
20.80
自引率
19.30%
发文量
2013
审稿时长
12 days
期刊介绍: Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.
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