{"title":"Impact of carrier capacitance on Geobacter enrichment and direct interspecies electron transfer under anaerobic conditions.","authors":"Shujuan Liu, Dandan Liang, Yixi Wang, Weihua He, Yujie Feng","doi":"10.1016/j.biortech.2025.132079","DOIUrl":null,"url":null,"abstract":"<p><p>Direct interspecies electron transfer (DIET) enhances anaerobic digestion by facilitating electron exchange between electroactive bacteria and methanogenic archaea. While Geobacter species are recognized for donating electrons to methanogens via DIET, they are rarely detected in mixed microbial communities. This study examined various non-electrode biological carriers (zeolite, carbon cloth, activated carbon and biochar) to promote Geobacter cultivation under anaerobic conditions and identify pivotal factors influencing their symbiosis with methanogens. Capacitive materials, such as activated carbon and biochar, significantly enriched Geobacter populations and strengthened DIET-based mutualism with Methanosarcina, both in the presence and absence of electric fields. Partial least-squares path modeling revealed that the porous structure and functional groups of materials positively and directly influenced the abundance of Geobacter and Methanosarcina. These findings contribute to a deeper understanding of critical properties of capacitive materials for screening functional microorganisms and guiding the design of electroactive materials to augment anaerobic treatment processes.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"419 ","pages":"132079"},"PeriodicalIF":9.7000,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioresource Technology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1016/j.biortech.2025.132079","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURAL ENGINEERING","Score":null,"Total":0}
引用次数: 0
Abstract
Direct interspecies electron transfer (DIET) enhances anaerobic digestion by facilitating electron exchange between electroactive bacteria and methanogenic archaea. While Geobacter species are recognized for donating electrons to methanogens via DIET, they are rarely detected in mixed microbial communities. This study examined various non-electrode biological carriers (zeolite, carbon cloth, activated carbon and biochar) to promote Geobacter cultivation under anaerobic conditions and identify pivotal factors influencing their symbiosis with methanogens. Capacitive materials, such as activated carbon and biochar, significantly enriched Geobacter populations and strengthened DIET-based mutualism with Methanosarcina, both in the presence and absence of electric fields. Partial least-squares path modeling revealed that the porous structure and functional groups of materials positively and directly influenced the abundance of Geobacter and Methanosarcina. These findings contribute to a deeper understanding of critical properties of capacitive materials for screening functional microorganisms and guiding the design of electroactive materials to augment anaerobic treatment processes.
期刊介绍:
Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies.
Topics include:
• Biofuels: liquid and gaseous biofuels production, modeling and economics
• Bioprocesses and bioproducts: biocatalysis and fermentations
• Biomass and feedstocks utilization: bioconversion of agro-industrial residues
• Environmental protection: biological waste treatment
• Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.