A Bioengineered Model of the Human Cornea for Preclinical Assessment of Human Ocular Exposure to Environmental Toxicants

IF 10 2区 医学 Q1 ENGINEERING, BIOMEDICAL
Se-jeong Kim, Ning Guo, Zong Yao Tan, Xing Gao, Pouria Fattahi, Haijiao Liu, Jeehan Chang, Mousa Younesi, Selice Jung, Yewhan Chung, Minkyung Song, Michelle Jung, Dan Dongeun Huh
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Abstract

Here a bioengineered platform is introduced to investigate adverse effects of environmental materials on the human cornea. Using primary cells, this system is capable of reproducing the differentiated corneal epithelium and its underlying stroma in the human eye, which can then be treated with externally applied solid, liquid, or gaseous substances in a controlled manner and under physiologically relevant conditions. The proof-of-principle of how this system can be used to simulate human ocular exposure to different classes of environmental toxicants for direct visualization and quantitative analysis of their potential to induce acute corneal injury and inflammation is demonstrated. This model can also be further engineered to create an electromechanically actuated array of multiple human corneal tissues that can emulate spontaneous eye blinking. Using this advanced system, it is shown that blinking-like mechanical motions may play a protective role against adverse effects of environmental toxicants. This work yields an immediately deployable in vitro technology for screening ocular toxicity of existing and emerging environmental materials of various types and may enable the development of more realistic, human-relevant preclinical toxicology models complementary to traditional animal testing.

Abstract Image

人类角膜生物工程模型的临床前评估人眼暴露于环境毒物。
本文介绍了一个生物工程平台来研究环境材料对人类角膜的不利影响。使用原代细胞,该系统能够在人眼中再生分化的角膜上皮及其底层基质,然后可以在受控的方式和生理相关条件下用外部施加的固体、液体或气体物质处理。该系统如何用于模拟人眼暴露于不同类别的环境毒物中,以直接可视化和定量分析其诱导急性角膜损伤和炎症的潜力的原理证明。该模型还可以进一步设计,以创建一个由多个人类角膜组织组成的机电驱动阵列,可以模拟自发眨眼。利用这种先进的系统,表明类似眨眼的机械运动可能对环境有毒物质的不利影响起保护作用。这项工作产生了一种可立即部署的体外技术,用于筛选各种类型的现有和新兴环境材料的眼毒性,并可能使开发更现实的、与人类相关的临床前毒理学模型成为传统动物试验的补充。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Advanced Healthcare Materials
Advanced Healthcare Materials 工程技术-生物材料
CiteScore
14.40
自引率
3.00%
发文量
600
审稿时长
1.8 months
期刊介绍: Advanced Healthcare Materials, a distinguished member of the esteemed Advanced portfolio, has been dedicated to disseminating cutting-edge research on materials, devices, and technologies for enhancing human well-being for over ten years. As a comprehensive journal, it encompasses a wide range of disciplines such as biomaterials, biointerfaces, nanomedicine and nanotechnology, tissue engineering, and regenerative medicine.
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