"Effects of Redox Status on Immediate Hypericin-Mediated Photodynamic Therapy in Human Glioblastoma T98G Cell Line".

IF 4.3 3区 化学 Q2 CHEMISTRY, MULTIDISCIPLINARY
ACS Omega Pub Date : 2024-12-28 eCollection Date: 2025-01-14 DOI:10.1021/acsomega.4c08553
Camila Aparecida Errerias Fernandes Cardinali, Camila Fabiano de Freitas, Renato Sonchini Gonçalves, Flavia Amanda Pedroso de Morais, Juliana Nunes de Lima Martins, Yandara Akamine Martins, Jurandir Fernando Comar, Patrícia de Souza Bonfim-Mendonça, André Luiz Tessaro, Elza Kimura, Wilker Caetano, Noboru Hioka, Kellen Brunaldi, Maria Ida Ravanelli
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引用次数: 0

Abstract

Glioblastoma Multiforme (GBM) is one of the most aggressive types of brain tumor. GBM can modulate glutathione (GSH) levels and regulate cellular redox state, which can explain its high resistance to chemotherapeutic agents. Photodynamic therapy (PDT) is a selective, nontoxic, and minimally invasive treatment approved for many types of cancer. PDT leads to cell death mainly by promoting the generation of reactive oxygen species (ROS). Thus, in the current study, PDT with the photosensitizer hypericin (Hyp), formulated in mixed 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)/biotinylated-pluronic F127 (F127-B) liposomes, in combination with the GSH synthesis inhibitor buthionine sulfoximine (BSO) were tested against T98G cell line of human glioblastoma. The mixed liposome was effective in delivering Hyp to the cells, leading to a dose relationship between Hyp and ROS levels. BSO potentiated Hyp cell uptake, decreased GSH levels regardless of Hyp concentration, and intensified ROS generation for 1.00 and 5.00 μmol L-1 Hyp. Nonetheless, cell death was more pronounced in the groups not treated with BSO, indicating that reduced GSH levels are not a decisive factor in achieving the PDT effects of Hyp. In conclusion, the mixed DPPC/F127-B liposomes were effective as a delivery system for Hyp. However, the combination of BSO and Hyp was not capable of optimizing PDT against T98G cells.

氧化还原状态对人胶质母细胞瘤T98G细胞系金丝桃素介导的即时光动力治疗的影响
多形性胶质母细胞瘤(GBM)是最具侵袭性的脑肿瘤之一。GBM可以调节谷胱甘肽(GSH)水平,调节细胞氧化还原状态,这可以解释其对化疗药物的高耐药。光动力疗法(PDT)是一种选择性的、无毒的、微创的治疗方法,被批准用于许多类型的癌症。PDT主要通过促进活性氧(ROS)的产生导致细胞死亡。因此,在本研究中,将光敏剂金丝桃素(Hyp)与GSH合成抑制剂丁硫氨酸亚砜(BSO)联合制成1,2-双棕榈酰- n-甘油-3-磷脂胆碱(DPPC)/生物素化-pluronic F127 (F127- b)脂质体,对PDT对人胶质母细胞瘤T98G细胞系的作用进行了实验。混合脂质体有效地将Hyp输送到细胞中,导致Hyp和ROS水平之间的剂量关系。BSO增强了Hyp的细胞摄取,降低了GSH水平,与Hyp浓度无关,并增强了1.00和5.00 μmol L-1 Hyp的ROS生成,但在未给予BSO的组中,细胞死亡更为明显,说明GSH水平降低不是Hyp产生PDT效果的决定性因素。BSO和Hyp的组合不能优化对T98G细胞的PDT。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Omega
ACS Omega Chemical Engineering-General Chemical Engineering
CiteScore
6.60
自引率
4.90%
发文量
3945
审稿时长
2.4 months
期刊介绍: ACS Omega is an open-access global publication for scientific articles that describe new findings in chemistry and interfacing areas of science, without any perceived evaluation of immediate impact.
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