InP-Based Quantum Dots as Photosensitizers in Photodynamic Antimicrobial Materials.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2025-02-17 Epub Date: 2025-01-16 DOI:10.1021/acsabm.4c01467

Lihan Chen, Chenyu Jiang, Frank Scholle, Alissa E Meo, Jun Ohata, Christopher B Gorman, Reza A Ghiladi

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引用次数: 0

Abstract

Ligand-functionalized InP-based quantum dots (QDs) have been developed as an innovative class of nontoxic photosensitizer suitable for antimicrobial applications, aimed at reducing or preventing pathogen transmission from one host to another via high contact surfaces. A hot injection method followed by functionalization via ligand exchange with 9-anthracene carboxylic acid (ACA) yielded the desired core/shell InP/ZnSe/ZnS QDs. Transmission electron microscopy (TEM) revealed these QDs to be uniform in size (∼3.2 nm), with light absorption across the entire visible spectrum (λ_max ∼550 nm). Under light excitation at 550 nm, the generation of singlet oxygen (¹O₂) was evidenced by its characteristic phosphorescence signal at 1278 nm, indicating successful energy transfer from the QDs to surface-anchored ACA ligands, in accordance with a type II mechanism for a photodynamically generated singlet oxygen. The InP/ZnSe/ZnS core/shell QDs were applied to cellulose via dip coating, and the resultant QDs-loaded material was assessed for antimicrobial photodynamic inactivation (aPDI) of both Gram-positive [methicillin-resistant Staphylococcus aureus (MRSA; ATCC-44), vancomycin-resistant Enterococcus faecium (VRE; ATCC-2320)] and Gram-negative [multidrug-resistant Acinetobacter baumannii (MDRAB; ATCC-1605), NDM-1 positive Klebsiella pneumoniae (KP; ATCC-2146)] bacteria under illumination (400-700 nm; 85 mW/cm²; 90 min). The highest inactivation was observed for MRSA, achieving at least 99.999% inactivation (5 log units). Antiviral photodynamic inactivation on human coronavirus 229E (HCoV-229E) and feline calicivirus (FCV) demonstrated complete viral inactivation (to the detection limit). Cytotoxicity studies showed that the QDs are nontoxic to mammalian cells in the dark. Together, these results confirm the promising potential of ligand-functionalized InP-based QDs to be employed as nontoxic photosensitizers as materials in self-sterilizing surfaces.

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基于inp的量子点作为光动力抗菌材料的光敏剂。

基于配体功能化inp的量子点（QDs）是一种创新的无毒光敏剂，适用于抗菌应用，旨在减少或防止病原体通过高接触面从一个宿主传播到另一个宿主。通过与9-蒽羧酸（ACA）交换配体进行功能化的热注入方法，得到了所需的核心/壳层InP/ZnSe/ZnS量子点。透射电子显微镜（TEM）显示，这些量子点在尺寸上是均匀的（~ 3.2 nm），在整个可见光谱（λmax ~ 550 nm）上具有光吸收。在550nm光激发下，单重态氧（1O2）在1278 nm处产生了特有的磷光信号，表明能量从量子点成功转移到表面锚定的ACA配体上，符合光动力生成单重态氧的II型机制。将InP/ZnSe/ZnS核/壳量子点通过浸渍涂覆在纤维素上，并对所得到的量子点负载材料进行了革兰氏阳性[耐甲氧西林金黄色葡萄球菌(MRSA；ATCC-44)，耐万古霉素屎肠球菌(VRE；ATCC-2320)和革兰氏阴性[耐多药鲍曼不动杆菌(MDRAB；ATCC-1605)， NDM-1阳性肺炎克雷伯菌(KP；ATCC-2146)]细菌在光照下(400-700 nm；85 mW / cm2;90分钟)。MRSA的失活率最高，至少达到99.999%（5个对数单位）。对人冠状病毒229E （HCoV-229E）和猫杯状病毒（FCV）的抗病毒光动力失活表现为完全病毒失活（达到检测限）。细胞毒性研究表明，量子点在黑暗中对哺乳动物细胞无毒。总之，这些结果证实了配体功能化的inp基量子点作为无毒光敏剂作为自杀菌表面材料的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.