MAO-B-triggered reaction for an optical triple-signal assay and AND logic gate application based on PEI-functionalized silver nanoparticles

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-01-21 DOI:10.1007/s00604-024-06908-x

Xin-Ru Zhang, Tian Chai, Xiang-Lei Chang, Ying Zhang, Cheng-Bo Wang, Song Liu, Yves Iradukunda, Xian-Yi Shang, Yong-Hong Cao, Wei-Feng Wang, Jun-Li Yang, Yin Qiang

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引用次数: 0

Abstract

A novel analytical method was designed and developed that exhibited ultraviolet–visible (UV–Vis), fluorescence (FL), and resonance Rayleigh scattering (RRS) signals for straightforward and comprehensive determination of monoamine oxidase B (MAO-B) using polyethylenimine-functionalized silver nanoparticles (PEI-Ag NPs). Through a facile one-step experiment, and NaOH assisted, in an aqueous solution of 100 ℃ for 40 min PEI reacted with AgNO₃ to generate PEI-Ag NPs with a yellow color and weak blue fluorescence. Interestingly, phenylacetaldehyde (PAA), a specific product of MAO-B, causes significant enhancement of the three optical signals of UV–Vis, FL, and RRS. A triple-signal readout sensing system was designed and constructed for MAO-B concentration assay. The limit of detection (LOD) was 0.42 (UV–Vis), 3.49 (FL), and 1.97 (RRS) μg/mL. AND logic gate analysis was applied to enhance its accurate identification. Eventually, the feasibility of the method was assessed by measuring MAO-B amount in human serum samples with satisfying recoveries. The method provided a new concept for the clinical detection of MAO-B.

Graphical abstract

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mao - b触发的光学三信号分析反应和基于pei功能化纳米银的与逻辑门应用

设计并开发了一种新的分析方法，该方法具有紫外-可见（UV-Vis），荧光（FL）和共振瑞利散射（RRS）信号，用于使用聚乙烯亚胺功能化银纳米粒子（PEI-Ag NPs）直接和全面地测定单胺氧化酶B （MAO-B）。通过简单的一步实验，在NaOH辅助下，PEI与AgNO3在100℃的水溶液中反应40 min，生成颜色为黄色，荧光为弱蓝色的PEI- ag NPs。有趣的是，苯乙醛（PAA）是MAO-B的一种特异性产物，可显著增强UV-Vis、FL和RRS三种光信号。设计并构建了用于MAO-B浓度测定的三信号读出传感系统。检出限（LOD）分别为0.42 （UV-Vis）、3.49 （FL）、1.97 (RRS) μg/mL。采用与逻辑门分析，提高了其识别的准确性。最后，通过测定人血清样品中MAO-B的含量来评价该方法的可行性，回收率令人满意。该方法为临床检测MAO-B提供了新的思路。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.