Smartphone-based non-invasive detection of salivary uric acid based on the fluorescence quenching of gleditsia sinensis carbon dots

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-01-21 DOI:10.1007/s00604-025-06949-w

Yujuan Xiang, Hongpeng Yu, Kegang Wu, Ziting Quan, Dong He, Cansheng Ou, Xianghua Chai, Yinglin Liang, Xuejuan Duan, Xiangyu Zhang, Qin Zha, Wei Xie

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Abstract

A smartphone-based non-invasive method was developed for salivary uric acid detection using Gleditsia Sinensis carbon dots (GS-CDs). The GS-CDs synthesized by the one-pot hydrothermal method emitted blue fluorescence at a maximum excitation wavelength of 350 nm and had good fluorescence stability in the presence of different ions, while showing selectivity to uric acid solution. The ability of uric acid (UA) to quench the fluorescent substances present in the GS-CDs, was confirmed through HPLC-FLD and LC–MS, FTIR and XPS. The results showed that UA reacted with GS-CDs, with a decrease in hydroxyl groups and the formation of carboxyl groups. The fluorescence quenching suggested a possible dynamic quenching mechanism. In addition, a smartphone-based non-invasive detection method was developed for the detection of salivary UA levels, which reflects blood UA levels. This study provides a new perspective on the utilization of GS shells and advances the development of non-invasive testing for UA.

Graphical Abstract

Abstract Image

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基于皂角碳点荧光猝灭的智能手机无创唾液尿酸检测

建立了一种基于智能手机的皂角碳点（GS-CDs）唾液尿酸检测方法。一锅水热法合成的GS-CDs在最大激发波长350 nm处发出蓝色荧光，在不同离子存在下具有良好的荧光稳定性，同时对尿酸溶液有选择性。通过HPLC-FLD、LC-MS、FTIR和XPS证实了尿酸（UA）猝灭GS-CDs中存在的荧光物质的能力。结果表明，UA与GS-CDs发生反应，羟基减少，羧基生成。荧光猝灭提示了一种可能的动态猝灭机制。此外，开发了一种基于智能手机的无创检测方法，用于检测唾液UA水平，反映血液UA水平。本研究为GS壳的利用提供了新的视角，促进了UA无创检测的发展。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.