Tachykinin regulates the innate immunity and disease resistance in Scylla paramamosain

IF 2.2 3区 农林科学 Q2 FISHERIES
Yong Wang, Fei Zhu
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引用次数: 0

Abstract

Tachykinin, a class of neuropeptides known for their direct interaction with vascular endothelial cells, plays a role in several essential physiological processes. This study aimed to investigate the role of tachykinin in the innate immunity of crustaceans, with a specific focus on hemocyte proliferation. RNA interference technology was employed to knock down tachykinin expression in Scylla paramamosain using tachykinin-dsRNA. The real-time quantitative PCR results demonstrated that the expression levels of proPO, β-actin, astakine, crustin antimicrobial peptide (CAP), JAK, and STAT were downregulated following the knockdown of tachykinin, but myosin, toll-like receptor (TLR), and MCM7 expressions were upregulated. Following tachykinin knockdown, the enzyme activities of phenoloxidase (PO), peroxidase (POD), lysozyme (LZM), acid phosphatase (ACP), catalase (CAT), and total hemocyte counts were reduced in S. paramamosain. The flow cytometry data indicated that tachykinin knockdown enhanced the phagocytosis and apoptosis rate of crab hemocytes and decreased hemocyte proliferation. And survival analysis showed that tachykinin knockdown increased the mortality rate of S. paramamosain post-infection with Vibrio alginolyticus. These findings suggest that tachykinin may play a regulatory role in hemocyte proliferation and influence the innate immune response in S. paramamosain. Additionally, tachykinin significantly affected the survival rate of crabs even in the absence of V. alginolyticus challenge, suggesting its vital role for the survival of S. paramamosain. In conclusion, our study provides further insights into the important role of tachykinin in the innate immunity and hemocyte proliferation of S. paramamosain.

Abstract Image

速激肽调节Scylla paramamosain的先天免疫和抗病能力
速激肽是一类与血管内皮细胞直接相互作用的神经肽,在几个重要的生理过程中起作用。本研究旨在探讨速激肽在甲壳类动物先天免疫中的作用,并特别关注血细胞增殖。采用RNA干扰技术,利用tachykinin- dsrna敲低Scylla paramamosain中tachykinin的表达。实时荧光定量PCR结果显示,在敲低速激肽后,proPO、β-actin、astakine、壳蛋白抗菌肽(CAP)、JAK和STAT的表达水平下调,而myosin、toll样受体(TLR)和MCM7的表达水平上调。抑制速激肽后,S. paramamosain的酚氧化酶(PO)、过氧化物酶(POD)、溶菌酶(LZM)、酸性磷酸酶(ACP)、过氧化氢酶(CAT)活性和总血细胞计数均降低。流式细胞术结果表明,敲低速激肽可提高蟹血细胞的吞噬率和凋亡率,抑制蟹血细胞的增殖。生存分析显示,敲低速激肽可提高S. paramamosain感染溶藻弧菌后的死亡率。这些发现提示速激肽可能在S. paramamosain的血细胞增殖和先天免疫反应中起调节作用。此外,即使在没有溶藻弧菌攻击的情况下,速激肽也显著影响螃蟹的存活率,这表明它对S. paramamosain的存活起着至关重要的作用。总之,我们的研究进一步揭示了速激肽在S. paramamosain先天免疫和血细胞增殖中的重要作用。
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来源期刊
Aquaculture International
Aquaculture International 农林科学-渔业
CiteScore
5.10
自引率
6.90%
发文量
204
审稿时长
1.0 months
期刊介绍: Aquaculture International is an international journal publishing original research papers, short communications, technical notes and review papers on all aspects of aquaculture. The Journal covers topics such as the biology, physiology, pathology and genetics of cultured fish, crustaceans, molluscs and plants, especially new species; water quality of supply systems, fluctuations in water quality within farms and the environmental impacts of aquacultural operations; nutrition, feeding and stocking practices, especially as they affect the health and growth rates of cultured species; sustainable production techniques; bioengineering studies on the design and management of offshore and land-based systems; the improvement of quality and marketing of farmed products; sociological and societal impacts of aquaculture, and more. This is the official Journal of the European Aquaculture Society.
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