Multifunctional MnO2 nanosheet based DNA tetrahedron- photosensitizer nanoplatform for gene-photodynamic modulation

IF 13.3 1区工程技术 Q1 ENGINEERING, CHEMICAL

Chemical Engineering Journal Pub Date : 2025-01-22 DOI:10.1016/j.cej.2025.159807

Yanlin Zheng, Fei Li, Xiaohui Zhu, Min Wang, Yuantao Wen, Tao Zeng, Wukun Zhong, Chao Xing, Chunhua Lu

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引用次数: 0

Abstract

Photodynamic therapy (PDT) holds great promise for treating various types of cancer. However, its clinical advancement is hindered by the intrinsic hypoxic tumor microenvironment, which reduces PDT efficacy, and safety concerns related to the biological specificity of photosensitizers. Here we constructed a MnO₂ nanosheet based DNA tetrahedron-photosensitizer (TMPyP4) nanoplatform (TPM), which integration with tumor targeting (aptamer), gene drugs (DNAzyme) and photosensitizer agents for enhanced PDT. Once the TPM entered cancer cells through aptamer targeting, the MnO₂ nanosheet reacts with endogenous acidic and H₂O₂ to generate Mn²⁺ and oxygen (O₂), thereby alleviating the tumor hypoxic. Subsequently, TMPyP4 further converts the generated O₂ into singlet oxygen (¹O₂) under near-infrared light, enhancing PDT efficacy both in vitro and in vivo. Specifically, the generated Mn²⁺ activates the DNAzyme and accelerate tumor target mRNA cleavage, resulting in effective PDT-gene synergistic therapy for tumors. This work offers novel perspectives on advancing precise and highly effective gene-PDT systems.

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基于多功能二氧化锰纳米片的DNA四面体光敏剂纳米平台的基因光动力调制

光动力疗法（PDT）在治疗各种类型的癌症方面具有很大的前景。然而，其临床进展受到肿瘤固有缺氧微环境的阻碍，这降低了PDT的疗效，以及与光敏剂的生物特异性相关的安全性问题。本文构建了一种基于MnO2纳米片的DNA四面体光敏剂（TMPyP4）纳米平台（TPM），该平台与肿瘤靶向（适体）、基因药物（DNAzyme）和光敏剂相结合，增强了PDT。TPM通过适体靶向进入癌细胞后，MnO2纳米片与内源性酸和H2O2反应生成Mn2+和氧（O2），从而缓解肿瘤缺氧。随后，TMPyP4在近红外光下进一步将生成的O2转化为单线态氧（1O2），增强PDT在体内和体外的效果。具体来说，产生的Mn2+激活DNAzyme，加速肿瘤靶mRNA的切割，从而有效地对肿瘤进行pdt基因协同治疗。这项工作为推进精确和高效的基因- pdt系统提供了新的视角。

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来源期刊

Chemical Engineering Journal 工程技术-工程：化工

CiteScore

21.70

自引率

9.30%

发文量

6781

审稿时长

2.4 months

期刊介绍： The Chemical Engineering Journal is an international research journal that invites contributions of original and novel fundamental research. It aims to provide an international platform for presenting original fundamental research, interpretative reviews, and discussions on new developments in chemical engineering. The journal welcomes papers that describe novel theory and its practical application, as well as those that demonstrate the transfer of techniques from other disciplines. It also welcomes reports on carefully conducted experimental work that is soundly interpreted. The main focus of the journal is on original and rigorous research results that have broad significance. The Catalysis section within the Chemical Engineering Journal focuses specifically on Experimental and Theoretical studies in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. These studies have industrial impact on various sectors such as chemicals, energy, materials, foods, healthcare, and environmental protection.