Distribution of the four type VI secretion systems in Pseudomonas aeruginosa and classification of their core and accessory effectors

IF 14.7 1区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Antonia Habich, Verónica Chaves Vargas, Luca A. Robinson, Luke P. Allsopp, Daniel Unterweger
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引用次数: 0

Abstract

Bacterial type VI secretion systems (T6SSs) are puncturing molecular machines that transport effector proteins to kill microbes, manipulate eukaryotic cells, or facilitate nutrient uptake. How and why T6SS machines and effectors differ within a species is not fully understood. Here, we applied molecular population genetics to the T6SSs in a global population of the opportunistic pathogen Pseudomonas aeruginosa. We reveal varying occurrence of up to four distinct T6SS machines. Moreover, we define conserved core T6SS effectors, likely critical for the biology of P. aeruginosa, and accessory effectors that can exhibit mutual exclusivity between strains. By ancestral reconstruction, we observed dynamic changes in the gain and loss of effector genes in the species’ evolutionary history. Our work highlights the potential importance of T6SS intraspecific diversity in bacterial ecology and evolution.

Abstract Image

铜绿假单胞菌四种VI型分泌系统的分布及其核心和辅助效应物的分类
细菌VI型分泌系统(t6ss)是一种刺穿性分子机器,可运输效应蛋白来杀死微生物、操纵真核细胞或促进营养摄取。T6SS机器和效应器如何以及为什么在一个物种中不同还没有完全了解。在这里,我们将分子群体遗传学应用于机会致病菌铜绿假单胞菌全球群体中的t6ss。我们揭示了多达四种不同的T6SS机器的不同发生。此外,我们定义了保守的核心T6SS效应物,可能对铜绿假单胞菌的生物学至关重要,以及在菌株之间表现出互斥性的附属效应物。通过祖先重建,我们观察到了物种进化史中效应基因的获得和损失的动态变化。我们的工作强调了T6SS种内多样性在细菌生态学和进化中的潜在重要性。
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来源期刊
Nature Communications
Nature Communications Biological Science Disciplines-
CiteScore
24.90
自引率
2.40%
发文量
6928
审稿时长
3.7 months
期刊介绍: Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.
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