Immobilization of ammonia-oxidizing bacteria using mycelial pellets: Preparation, characteristics, and application for nitritation

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2025-01-15 DOI:10.1016/j.biortech.2025.132083

Ming Chen, Lei Jin, Xiaoying Liu, Renjie Li, Huiling Xian, Chao Guo

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引用次数: 0

Abstract

Ammonia-oxidizing bacteria (AOB) sourced from an aerobic granular sludge (AGS) process were rapidly enriched by progressively increasing ammonia nitrogen (NH4+-N) loads, achieving a Nitrosomonas abundance of 20.7 % and a nitrite accumulation rate exceeding 80 %. Mycelial pellets formed by Cladosporium, isolated from the same AGS system, provided a porous surface structure for the immobilization of the enriched AOB, creating mycelial pellet/AOB composites. Robust microbial colonization and aggregation in mycelial pellet porous matrix were facilitated by a higher level of extracellular polymeric substances (EPS) compared to conventional AGS. Static tests showed a maximum NH4+-N oxidation rate of 17.7 mg/(gMLVSS·h), higher than free AOB (8.5 mg/(gMLVSS·h)). In multi-recycling tests, the composites maintained 96.6 % NH4+-N oxidation, demonstrating superior repeatability and stability. The results highlight advantages of mycelial pellets as biocompatible carriers in immobilizing AOB sourced from the same system, offering insights into improved nitritation performance and durability, making them promising for practical wastewater treatment.

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.