Efficiently radiation-induced degradation and extraction of Polygonatum sibiricum polysaccharides and its excellent ROS scavenging properties

Abstract

The traditional Chinese medicine Polygonatum sibiricum (PS) possessed anti-inflammatory and reactive oxygen species (ROS) scavenging properties. Currently, the extraction method for Polygonatum sibiricum polysaccharides primarily relies on traditional techniques, which exhibit low extraction efficiency and limit ROS scavenging activity. The present study aims to enhance the then-current low extraction yield and poor activity of polygonatum polysaccharide by innovating the technology of electron beam radiation extraction and degradation cycle. The optimal radiation extraction process for obtaining Polygonatum sibiricum crude polysaccharide (PSCP) was established using a combination of single factor and response surface methods through the radiation extraction technique. The Polygonatum sibiricum polysaccharides (PSP) was obtained through the process of isolation and purification, while the RPSP was acquired by means of radiation-induced degradation. The GPC, FT-IR, XRD, and SEM techniques were employed for their characterization. The bioactivity test demonstrated the excellent biocompatibility of radiolytic Polygonatum sibiricum polysaccharides (RPSP). In vitro and intracellular ROS scavenging assays revealed that RPSP exhibited superior ROS scavenging ability, RPSP-2 having the highest efficacy at a molecular weight of 201KDa. The above studies demonstrated that RPSP-2, obtained through electron beam radiation extraction and degradation cycle technology, could be effectively utilized for ROS scavenging in the context of oxidative inflammation, thereby playing a significant therapeutic role.