Aging-dependent temporal regulation of MIR156 epigenetic silencing by CiLDL1 and CiNF-YB8 in chrysanthemum

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2025-01-17 DOI:10.1111/nph.20354

Xuekai Gao, Lei Liu, Tianle Wang, Chuyan Jiang, Yujin Xue, Yahui Sun, Zhaoyu Gu, Yanjie Xu, Cai-Zhong Jiang, Junping Gao, Bo Hong, Chao Ma

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引用次数: 0

Abstract

Temporal decline in microRNA miR156 expression is crucial for the transition to, and maintenance of, the adult phase and flowering competence in flowering plants. However, the molecular mechanisms underlying the temporal regulation of miR156 reduction remain largely unknown.
Here, we investigated the epigenetic mechanism regulating the temporal silencing of cin-MIR156 in wild chrysanthemum (Chrysanthemum indicum), focusing on the role of the lysine-specific demethylase CiLDL1 and the nuclear factor Y complex.
CiLDL1 and CiNF-YB8 interact with the classical histone-like fold domain (HFD) of CiNF-YC1 and CiNF-YA3, which form distinct heterotrimers binding to the ‘CCAAT’ box in the promoter region of cin-MIR156ab. CiLDL1 and CiNF-YB8 have opposing effects on cin-MIR156ab expression, with influencing histone 3 lysine 4 demethylation (H3K4me2) levels at the cin-MIR156ab locus. During aging, decreased CiNF-YB8 expression leads to a quantitative switch from the CiNF-YA3–CiNF-YC1–CiNF-YB8 heterotrimer to the CiNF-YA3–CiNF-YC1–CiLDL1 heterotrimer, which reduces H3K4me2 levels at the cin-MIR156ab locus, thus temporal silencing its expression.
Our results thus reveal that the dynamic regulatory shift between CiLDL1 and CiNF-YB8 ensures proper aging-dependent flowering in chrysanthemum.

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菊花中CiLDL1和cif - yb8对MIR156表观遗传沉默的衰老依赖性时间调控

microRNA miR156的表达在时间上的下降对于开花植物进入和维持成虫期和开花能力至关重要。然而，miR156减少的时间调控的分子机制在很大程度上仍然未知。在此，我们研究了野菊花（chrysanthemum indicum）中调节cin-MIR156时间沉默的表观遗传机制，重点研究了赖氨酸特异性去甲基化酶CiLDL1和核因子Y复合物的作用。CiLDL1和cif - yb8与cif - yc1和cif - ya3的经典组蛋白样折叠结构域（HFD）相互作用，形成不同的异源三聚体，与cin-MIR156ab启动子区域的“CCAAT”盒子结合。CiLDL1和cif - yb8对cin-MIR156ab的表达有相反的影响，影响cin-MIR156ab位点的组蛋白3赖氨酸4去甲基化（H3K4me2）水平。在衰老过程中，cif - yb8表达的减少导致从cif - ya3 - cif - yc1 - cif - yb8异源三聚体向cif - ya3 - cif - yc1 - cildl1异源三聚体的数量转换，从而降低了cin-MIR156ab位点的H3K4me2水平，从而使其表达暂时沉默。因此，我们的研究结果表明，CiLDL1和cif - yb8之间的动态调控转移确保了菊花适当的衰老依赖性开花。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.