Intramolecular DNA Wheel Construction for Highly Sensitive Electrochemical Detection of miRNA

IF 9.1 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2025-01-15 DOI:10.1021/acs.nanolett.4c05127

Xiaolin Qu, Yiwei Han, Qiuyan Huang, Peng Miao

引用次数: 0

Abstract

Accurate and reliable quantification of disease-related biomolecules is essential for clinical diagnosis. In this study, a novel electrochemical approach is developed based on a target triggered DNA nanostructural switch from a hairpin dimer to a double-stranded wheel. During the process, electrochemical species get closer to the electrode interface, and the multiple intramolecular strand displacements are beneficial to low abundant target analysis. In addition, the use of nicking endonuclease mitigates background interference. This strategy enables highly sensitive and selective quantification of the target miRNA. A linear relationship is established between the peak current intensity and the logarithm of miRNA concentration within the range from 0.1 to 20 fM. This method also demonstrates high accuracy in the analysis of clinical samples, holding great potential for DNA nanotechnology in diagnostic applications.

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高灵敏度电化学检测miRNA的分子内DNA轮结构

准确可靠地量化与疾病相关的生物分子对临床诊断至关重要。本研究基于目标触发的 DNA 纳米结构从发夹二聚体到双链轮的转换，开发了一种新型电化学方法。在这一过程中，电化学物种更接近电极界面，分子内的多重链位移有利于低丰度目标分析。此外，使用核酸内切酶可减轻背景干扰。这种策略可实现对目标 miRNA 的高灵敏度和选择性定量。在 0.1 至 20 fM 范围内，峰值电流强度与 miRNA 浓度的对数呈线性关系。这种方法在分析临床样本时也表现出很高的准确性，为 DNA 纳米技术在诊断方面的应用提供了巨大的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.