Prenatal ambient temperature exposure and cord blood and placental mitochondrial DNA content: Insights from the ENVIRONAGE birth cohort study

IF 10.3 1区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environment International Pub Date : 2025-02-01 DOI:10.1016/j.envint.2025.109267

Eleni Renaers , Congrong Wang , Esmée M. Bijnens , Michelle Plusquin , Tim S. Nawrot , Dries S. Martens

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引用次数: 0

Abstract

Background

Mitochondrial DNA content (mtDNAc) at birth is a sensitive biomarker to environmental exposures that may play an important role in later life health. We investigated sensitive time windows for the association between prenatal ambient temperature exposure and newborn mtDNAc.

Methods

In the ENVIRONAGE birth cohort (Belgium), we measured cord blood and placental mtDNAc in 911 participants using a quantitative real-time polymerase chain reaction. We associated newborn mtDNAc with average weekly mean temperature during pregnancy using distributed lag nonlinear models (DLNMs). Double-threshold DLNMs were used to study the relationships between ambient temperature and mtDNAc below predefined low (5th, 10th, 15th percentile of the temperature distribution) and above predefined high temperature thresholds (95th, 90th, 85th percentile of the temperature distribution).

Findings

Prenatal temperature exposure above the used high temperature thresholds was linked to lower cord blood mtDNAc, with the strongest effect in trimester 2 (cumulative estimates ranging from −21.4% to −25.6%). Placental mtDNAc showed positive and negative associations for high temperature exposure depending on the applied high temperature threshold. Negative associations were observed during trimester 1 using the 90th and 95th percentile threshold (−26.1% and –33.2% lower mtDNAc respectively), and a positive association in trimester 3 when applying the most stringent 95th percentile threshold (127.0%). Low temperature exposure was associated with higher mtDNAc for both cord blood and placenta. Cord blood mtDNAc showed a positive association in trimester 2 when using the 10th percentile threshold (11.3%), while placental mtDNAc showed positive associations during the whole gestation and for all applied thresholds (estimates ranging from 80.8% − 320.6%).

Interpretation

Our study shows that in utero temperature exposure is associated with differences in newborn mtDNAc at birth, with stronger associations observed in the placenta. These findings highlight the impact of prenatal ambient temperature exposure on mtDNAc during pregnancy.

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产前环境温度暴露与脐带血和胎盘线粒体DNA含量：来自ENVIRONAGE出生队列研究的见解

出生时线粒体DNA含量（mtDNAc）是对环境暴露敏感的生物标志物，可能在以后的生活健康中发挥重要作用。我们研究了产前环境温度暴露与新生儿mtDNAc之间关系的敏感时间窗。方法在ENVIRONAGE出生队列（比利时）中，我们使用定量实时聚合酶链反应测量了911名参与者的脐带血和胎盘mtDNAc。我们使用分布滞后非线性模型（DLNMs）将新生儿mtDNAc与怀孕期间的周平均温度联系起来。双阈值dlnm评估环境温度与mtDNAc之间的线性关系，其低于和高于预定义的低（温度分布的第5、10、15百分位）和高温阈值（温度分布的第95、90、85百分位）。发现产前温度暴露高于使用的高温阈值与脐带血mtDNAc降低有关，在妊娠2个月的影响最大（累积估计范围为- 21.4%至- 25.6%）。根据应用的高温阈值，胎盘mtDNAc与高温暴露表现出正相关和负相关。使用第90和第95百分位阈值（mtDNAc分别降低- 26.1%和-33.2%）在妊娠1个月观察到负相关，使用最严格的第95百分位阈值（127.0%）在妊娠3个月观察到正相关。低温暴露与脐带血和胎盘中较高的mtDNAc有关。当使用第10百分位阈值时，脐带血mtDNAc在妊娠2期显示正相关（11.3%），而胎盘mtDNAc在整个妊娠期和所有应用阈值时显示正相关（估计范围为80.8% - 320.6%）。我们的研究表明，子宫内温度暴露与出生时新生儿mtDNAc的差异有关，在胎盘中观察到更强的相关性。这些发现强调了产前环境温度暴露对妊娠期间mtDNAc的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Environment International 环境科学-环境科学

CiteScore

21.90

自引率

3.40%

发文量

734

审稿时长

2.8 months

期刊介绍： Environmental Health publishes manuscripts focusing on critical aspects of environmental and occupational medicine, including studies in toxicology and epidemiology, to illuminate the human health implications of exposure to environmental hazards. The journal adopts an open-access model and practices open peer review. It caters to scientists and practitioners across all environmental science domains, directly or indirectly impacting human health and well-being. With a commitment to enhancing the prevention of environmentally-related health risks, Environmental Health serves as a public health journal for the community and scientists engaged in matters of public health significance concerning the environment.