A Mitochondria-targeted Iridium (Ⅲ) Complex-Based Sensor for endogenous GSH Detection in living Cells

IF 3.6 3区 化学 Q2 CHEMISTRY, ANALYTICAL
Analyst Pub Date : 2025-01-16 DOI:10.1039/d4an01465k
Sha Xu, Qikai Ju, Xueting Mao, Tangxuan Cai, Daobin Zhang
{"title":"A Mitochondria-targeted Iridium (Ⅲ) Complex-Based Sensor for endogenous GSH Detection in living Cells","authors":"Sha Xu, Qikai Ju, Xueting Mao, Tangxuan Cai, Daobin Zhang","doi":"10.1039/d4an01465k","DOIUrl":null,"url":null,"abstract":"Glutathione (GSH) plays an important role in maintaining REDOX homeostasis in biological systems. Development of reliable glutathione sensors is of great significance to better understand the role of biomolecules in living cells and organisms. Based on advantages of the photophysical properties of iridium complexes, we proposed a \"turn-on\" phosphorescent sensor. Ir-DNFB had the characteristics of large Stokes shift, high sensitivity to GSH detection, low cytotoxicity, and extremely short response time, which can specifically analyze glutathione in living cells and highly target endogenous glutathione in mitochondria. Due to the N-H portion on imidazole ring of Ir-DNFB could form a new electrostatic interaction with α-carboxyl group on glutamate moitey of glutathione, then the nucleophilic attack reaction was regulated by sulfydryl group on GSH, following the ether bond linking 2,4-dinitrobenzene to probe Ir-DNFB was broken, accompanied with phosphorescence enhancement. Most importantly, the process of recognizing glutathione was not affected by other amino acids. Overall, this work provided a very useful tool for rapidly distinguishing between normal, inflammatory, and progressive tumor cells.","PeriodicalId":63,"journal":{"name":"Analyst","volume":"9 1","pages":""},"PeriodicalIF":3.6000,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analyst","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1039/d4an01465k","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0

Abstract

Glutathione (GSH) plays an important role in maintaining REDOX homeostasis in biological systems. Development of reliable glutathione sensors is of great significance to better understand the role of biomolecules in living cells and organisms. Based on advantages of the photophysical properties of iridium complexes, we proposed a "turn-on" phosphorescent sensor. Ir-DNFB had the characteristics of large Stokes shift, high sensitivity to GSH detection, low cytotoxicity, and extremely short response time, which can specifically analyze glutathione in living cells and highly target endogenous glutathione in mitochondria. Due to the N-H portion on imidazole ring of Ir-DNFB could form a new electrostatic interaction with α-carboxyl group on glutamate moitey of glutathione, then the nucleophilic attack reaction was regulated by sulfydryl group on GSH, following the ether bond linking 2,4-dinitrobenzene to probe Ir-DNFB was broken, accompanied with phosphorescence enhancement. Most importantly, the process of recognizing glutathione was not affected by other amino acids. Overall, this work provided a very useful tool for rapidly distinguishing between normal, inflammatory, and progressive tumor cells.
求助全文
约1分钟内获得全文 求助全文
来源期刊
Analyst
Analyst 化学-分析化学
CiteScore
7.80
自引率
4.80%
发文量
636
审稿时长
1.9 months
期刊介绍: "Analyst" journal is the home of premier fundamental discoveries, inventions and applications in the analytical and bioanalytical sciences.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信