Exploring the potential of solid and liquid amniotic membrane biomaterial in 3D models for prostate cancer research: A comparative analysis with 2D models

IF 2.7 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-01-07 DOI:10.1016/j.tice.2025.102726

Keykavos Gholami , Mehrnaz Izadi , Ramin Heshmat , Seyed Mohammad Kazem Aghamir

{"title":"Exploring the potential of solid and liquid amniotic membrane biomaterial in 3D models for prostate cancer research: A comparative analysis with 2D models","authors":"Keykavos Gholami , Mehrnaz Izadi , Ramin Heshmat , Seyed Mohammad Kazem Aghamir","doi":"10.1016/j.tice.2025.102726","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>Research and tools are necessary for understanding prostate cancer biology. 3D cell culture models have been created to overcome the limitations of animal models and 2D cell culture. The amniotic membrane (AM), a natural biomaterial, emerges as an ideal scaffold for 3D cultures due to its accessibility and incorporation of the extracellular matrix (ECM) in both solid and liquid forms.</div></div><div><h3>Methods</h3><div>In this study, decellularized human amniotic membranes (DAM) and AM hydrogel were obtained and characterized. The solid DAM scaffold was employed to analyse cell proliferation, cell cycle, migration, apoptosis, and the content of epithelial-mesenchymal transition (EMT) proteins in prostate cancer cells in comparison to traditional 2D culture conditions under androgen deprivation treatment. Additionally, the liquid form of AM was assessed for its potential for 3D cultures of prostate cancer cells such as cells embedded in ECM, spheroid encapsulation, and invasion, with a parallel comparison to collagen.</div></div><div><h3>Results</h3><div>The 3D DAM scaffold significantly impacted cancer cell migration, morphology, proliferation, and EMT protein expression compared to 2D models. AM hydrogel effectively preserved the structural integrity of spheroids and led to lower proliferated cells embedded in AM hydrogel compared to 2D culture. AM hydrogel, like collagen, has the potential to be utilized for simulating in vitro cellular invasion from the ECM.</div></div><div><h3>Conclusion</h3><div>In summary, the potential of the biomaterial of DAM and AM hydrogel in creating 3D culture models, combined with the brief duration required for decellularizing the AM, suggests that these materials offer an ideal tool for in vitro prostate cancer research.</div></div>","PeriodicalId":23201,"journal":{"name":"Tissue & cell","volume":"93 ","pages":"Article 102726"},"PeriodicalIF":2.7000,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tissue & cell","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0040816625000060","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ANATOMY & MORPHOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Objective

Research and tools are necessary for understanding prostate cancer biology. 3D cell culture models have been created to overcome the limitations of animal models and 2D cell culture. The amniotic membrane (AM), a natural biomaterial, emerges as an ideal scaffold for 3D cultures due to its accessibility and incorporation of the extracellular matrix (ECM) in both solid and liquid forms.

Methods

In this study, decellularized human amniotic membranes (DAM) and AM hydrogel were obtained and characterized. The solid DAM scaffold was employed to analyse cell proliferation, cell cycle, migration, apoptosis, and the content of epithelial-mesenchymal transition (EMT) proteins in prostate cancer cells in comparison to traditional 2D culture conditions under androgen deprivation treatment. Additionally, the liquid form of AM was assessed for its potential for 3D cultures of prostate cancer cells such as cells embedded in ECM, spheroid encapsulation, and invasion, with a parallel comparison to collagen.

Results

The 3D DAM scaffold significantly impacted cancer cell migration, morphology, proliferation, and EMT protein expression compared to 2D models. AM hydrogel effectively preserved the structural integrity of spheroids and led to lower proliferated cells embedded in AM hydrogel compared to 2D culture. AM hydrogel, like collagen, has the potential to be utilized for simulating in vitro cellular invasion from the ECM.

Conclusion

In summary, the potential of the biomaterial of DAM and AM hydrogel in creating 3D culture models, combined with the brief duration required for decellularizing the AM, suggests that these materials offer an ideal tool for in vitro prostate cancer research.

查看原文本刊更多论文

探讨固体和液体羊膜生物材料在前列腺癌三维模型研究中的潜力：与二维模型的比较分析。

目的：了解前列腺癌生物学需要研究和工具。3D细胞培养模型已经创建，以克服动物模型和2D细胞培养的局限性。羊膜（AM）是一种天然的生物材料，由于其可及性和结合细胞外基质（ECM）的固体和液体形式，成为3D培养的理想支架。方法：制备脱细胞人羊膜（DAM）和AM水凝胶，并对其进行表征。采用固体DAM支架分析前列腺癌细胞的细胞增殖、细胞周期、迁移、凋亡和上皮-间质转化（EMT）蛋白含量，并与传统二维培养条件下雄激素剥夺处理进行比较。此外，我们还评估了AM液体形式在前列腺癌细胞三维培养中的潜力，如ECM内包埋细胞、球形包埋细胞和侵袭细胞，并与胶原蛋白进行了平行比较。结果：与2D模型相比，3D DAM支架显著影响癌细胞迁移、形态、增殖和EMT蛋白表达。AM水凝胶有效地保持了球体的结构完整性，与二维培养相比，AM水凝胶包埋的细胞增殖率较低。AM水凝胶与胶原蛋白一样，具有模拟体外细胞侵袭的潜力。结论：总之，DAM和AM水凝胶生物材料在创建3D培养模型方面的潜力，以及AM脱细胞所需的短时间，表明这些材料为体外前列腺癌研究提供了理想的工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.