Differential effects of chronic unpredictable stress on behavioral and molecular (cortisol and microglia-related neurotranscriptomic) responses in adult leopard (leo) zebrafish.

IF 2.5 3区农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Fish Physiology and Biochemistry Pub Date : 2025-01-15 DOI:10.1007/s10695-024-01446-y

Tatiana O Kolesnikova, Nikita O Prokhorenko, Sahil V Amikishiev, Vadim S Nikitin, Anton D Shevlyakov, Aleksey N Ikrin, Radmir R Mukhamadeev, Anastasiia D Buglinina, Kirill V Apukhtin, Anastasia M Moskalenko, Nikita P Ilyin, Murilo S de Abreu, Konstantin A Demin, Allan V Kalueff

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Abstract

Stress plays a key role in mental, neurological, endocrine, and immune disorders. The zebrafish (Danio rerio) is rapidly gaining popularity as s model organism in stress physiology and neuroscience research. Although the leopard (leo) fish are a common outbred zebrafish strain, their behavioral phenotypes and stress responses remain poorly characterized. Here, we examined the effects of a 5-week chronic unpredictable stress (CUS) exposure on adult leo zebrafish behavior, cortisol levels, and brain gene expression. Compared to their unstressed control leo counterparts, CUS-exposed fish showed paradoxically lower anxiety-like, but higher whole-body cortisol levels and altered expression of multiple pro- and anti-inflammatory brain genes. Taken together, these findings suggest that behavioral and physiological (endocrine and genomic) responses to CUS do differ across zebrafish strains. These findings add further complexity to systemic effects of chronic stress in vivo and also underscore the importance of considering the genetic background of zebrafish in stress research.

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慢性不可预测应激对成年斑马鱼行为和分子（皮质醇和小胶质细胞相关神经转录组学）反应的差异影响。

压力在精神、神经、内分泌和免疫紊乱中起着关键作用。斑马鱼（Danio rerio）作为应激生理学和神经科学研究的一种模式生物迅速受到欢迎。虽然豹鱼是一种常见的近亲繁殖的斑马鱼品种，但它们的行为表型和应激反应仍然缺乏特征。在这里，我们研究了5周的慢性不可预测应激（CUS）暴露对成年狮子座斑马鱼行为、皮质醇水平和大脑基因表达的影响。与没有压力的对照组相比，暴露于cu的鱼表现出较低的焦虑样，但全身皮质醇水平较高，多种促炎和抗炎脑基因的表达也发生了变化。综上所述，这些发现表明斑马鱼品系对CUS的行为和生理（内分泌和基因组）反应确实不同。这些发现进一步增加了体内慢性应激系统效应的复杂性，也强调了在应激研究中考虑斑马鱼遗传背景的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fish Physiology and Biochemistry 农林科学-生化与分子生物学

CiteScore

5.60

自引率

6.90%

发文量

106

审稿时长

4 months

期刊介绍： Fish Physiology and Biochemistry is an international journal publishing original research papers in all aspects of the physiology and biochemistry of fishes. Coverage includes experimental work in such topics as biochemistry of organisms, organs, tissues and cells; structure of organs, tissues, cells and organelles related to their function; nutritional, osmotic, ionic, respiratory and excretory homeostasis; nerve and muscle physiology; endocrinology; reproductive physiology; energetics; biochemical and physiological effects of toxicants; molecular biology and biotechnology and more.