Pathway of typical β-Lactam antibiotics degradation by black soldier fly and response characteristic of its intestinal microbes.

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2025-01-12 DOI:10.1016/j.biortech.2025.132067

Fang Li, Chen Wang, Zhengzheng Zhao, Chongrui Yang, Bingqi Gao, Ziniu Yu, Jibin Zhang, Minmin Cai, Chan Yu

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Abstract

To effectively address the contamination caused by antibiotic misuse, this study was conducted to enhance the removal of amoxicillin (AMX) and penicillin sodium (PEN) by incorporating black soldier fly larvae (BSFL). The results showed that BSFL increased the degradation rates of AMX and PEN to 71.00 % and 80.89 %, respectively, and shortened their half-lives to 238 h and 160 h. Proteobacteria (26.2 %-82.0 %), Firmicutes (13.3 %-54.0 %), Acinobacteriota (2.1 %-23.4 %), and Bacteroidota (1.3 %-10.1 %) were the intestinal dominant microorganisms during transformation. Five bacteria with β-lactam antibiotic resistance in the BSFL gut were isolated, among which Morganella morganii demonstrated strong antibiotic tolerance and high removal rates of AMX and PEN in both in vitro and in vivo experiments, ranging from 58.99 % to 95.87 %. BSFL intestinal bacteria disrupted the quaternary pharmacophore of AMX and PEN, breaking them down into at least seven and five metabolites, respectively.

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为了有效解决滥用抗生素造成的污染问题，本研究通过加入黑兵蝇幼虫（BSFL）来提高阿莫西林（AMX）和青霉素钠（PEN）的去除率。结果表明，BSFL 可将 AMX 和 PEN 的降解率分别提高至 71.00 % 和 80.89 %，并将其半衰期分别缩短至 238 小时和 160 小时。转化过程中的肠道优势微生物为蛋白细菌（26.2 %-82.0 %）、固着菌（13.3 %-54.0 %）、醋酸菌群（2.1 %-23.4 %）和类杆菌（1.3 %-10.1 %）。在 BSFL 肠道中分离出五种对β-内酰胺类抗生素具有耐药性的细菌，其中摩根氏摩根菌（Morganella morganii）在体外和体内实验中均表现出很强的抗生素耐受性，对 AMX 和 PEN 的去除率很高，从 58.99 % 到 95.87 % 不等。BSFL 肠道细菌破坏了 AMX 和 PEN 的四价药效团，将其分别分解成至少七种和五种代谢物。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.