Characterization of fluoranthene degradation by the novel isolated Pseudomonas xizangensis S4 and its application potential immobilized in potassium humate-modified biochar

Abstract

Enhanced microbial remediation represents a promising technique for the removal of polycyclic aromatic hydrocarbons (PAHs). However, high-efficiency remediation agents remain limited, including microbial resources and remediation materials. In this study, a novel strain of Pseudomonas xizangensis S4 was isolated from plateau lake sediment, exhibiting a fluoranthene degradation rate of 41.90 % at 50 ppm within 7 d. The key degradation genes identified through genomic and transcriptomic analyses included ndmC, dmpK, dmpB, and dmpH. The metabolites detected via GC–MS analysis were biphenyls, parabens, and phthalate esters. Based on the above results, the degradation mechanisms of fluoranthene were deduced. Furthermore, an efficient remediation agent was developed, utilizing potassium humate-modified biochar to immobilize bacterial cells. The developed remediation agent enhanced the removal efficiency by 16.71 % compared to the single strain. Thus, the application of potassium humate-modified biochar for the immobilization of P. xizangensis S4 represents a promising method for the remediation of PAH-contaminated soil.