A self-aggregated thermally activated delayed fluorescence nanoprobe for HClO imaging and activatable photodynamic therapy.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-05-01 Epub Date: 2025-01-10 DOI:10.1016/j.talanta.2025.127570

Qingyang Sha, Xinsheng Li, Xixin Gu, Tao Yuan, Jianli Hua

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引用次数: 0

Abstract

Hypochlorous acid (HClO/ClO^-) is a common ROS that exhibits elevated activity levels in cancer cells. In this study, an ClO^--triggered TADF probe, PTZ-MNI, was designed based on a naphthalimide core. PTZ-MNI self-assemble in aqueous environments, exhibiting significantly enhanced fluorescence that demonstrated typical aggregation-induced delayed fluorescence (AIDF) characteristics. The probe not only showed high sensitivity to ClO^- but also exhibited remarkable selectivity over other reactive oxygen species and disturbance. PTZ-MNI displayed TADF characteristic, including sensitivity to oxygen in toluene, insensitivity to oxygen in aggregated states that maintain long fluorescence lifetimes, a vertical conformation, and a minimal ΔE_ST of 0.01 eV. Cell imaging studies showed the probe could trace ClO^- by red to green fluorescence in HeLa cell. The colocalization analysis indicated its excellent lysosome-targeting specificity. In addition, PTZ-MNI-O, the compound after oxidation, exhibited effective ROS generation ability and significant PDT effect after irradiation. This work provides guidance for the rational design of responsive TADF luminescent materials used in cell imaging and activatable-PDT.

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一种用于HClO成像和可激活光动力治疗的自聚集热激活延迟荧光纳米探针。

次氯酸（HClO/ClO-）是一种常见的活性氧，在癌细胞中表现出较高的活性水平。在本研究中，设计了一种基于萘酰亚胺核的ClO触发TADF探针PTZ-MNI。PTZ-MNI在水环境中自组装，荧光显著增强，表现出典型的聚集诱导延迟荧光（AIDF）特征。该探针不仅对ClO-具有很高的灵敏度，而且对其他活性氧和干扰也有很好的选择性。PTZ-MNI表现出TADF特征，包括对甲苯中的氧敏感，对保持长荧光寿命的聚集态氧不敏感，垂直构象，最小ΔEST为0.01 eV。细胞成像研究表明，该探针可以通过HeLa细胞的红色到绿色荧光来追踪ClO-。共定位分析表明其具有良好的溶酶体靶向特异性。此外，氧化后的化合物PTZ-MNI-O在辐照后表现出有效的ROS生成能力和显著的PDT效应。这项工作为合理设计用于细胞成像和活化pdt的响应性TADF发光材料提供了指导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.